Structural analysis of the yeast SWI/SNF chromatin remodeling complex
Authors
Smith, Corey LewisHorowitz-Scherer, Rachel A.
Flanagan, Joan Frances
Woodcock, Christopher L.
Peterson, Craig L.
UMass Chan Affiliations
Program in Molecular MedicineProgram in Gene Function and Expression
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2003-01-14Keywords
Chromatin; DNA-Binding Proteins; Image Processing, Computer-Assisted; Iodine Radioisotopes; Macromolecular Substances; Microscopy, Electron, Scanning Transmission; Models, Molecular; Molecular Weight; *Nuclear Proteins; Protein Conformation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription FactorsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs.Source
Nat Struct Biol. 2003 Feb;10(2):141-5. Link to article on publisher's siteDOI
10.1038/nsb888Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32564PubMed ID
12524530Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/nsb888