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dc.contributor.authorSmith, Corey Lewis
dc.contributor.authorPeterson, Craig L.
dc.date2022-08-11T08:08:48.000
dc.date.accessioned2022-08-23T16:08:55Z
dc.date.available2022-08-23T16:08:55Z
dc.date.issued2003-08-02
dc.date.submitted2009-01-12
dc.identifier.citation<p>Methods. 2003 Sep;31(1):104-9.</p>
dc.identifier.issn1046-2023 (Print)
dc.identifier.doi10.1016/S1046-2023(03)00094-X
dc.identifier.pmid12893180
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32565
dc.description.abstractRapid protein purification methodologies, such as strategies involving the tandem affinity purification module, have resulted in the identification of a tremendous number of multisubunit protein complexes. Furthermore, in this modern genomic age, mass spectrometry methods are often coupled with affinity purification to identify the genes that encode each protein subunit. However, simple methodologies to determine the stoichiometry of individual subunits within a multisubunit complex have not received much attention. In this article we describe a procedure to rapidly and efficiently determine the stoichiometry of subunits within multisubunit complexes using a combination of tandem affinity purification and quantitative 125I labeling of subunit tyrosines.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=12893180&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/S1046-2023(03)00094-X
dc.subjectElectrophoresis, Polyacrylamide Gel; Fungal Proteins; Indicators and Reagents; Iodine Radioisotopes; Kinetics; Polymerase Chain Reaction; Radioisotope Dilution Technique; Tyrosine; Yeasts
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleCoupling tandem affinity purification and quantitative tyrosine iodination to determine subunit stoichiometry of protein complexes
dc.typeJournal Article
dc.source.journaltitleMethods (San Diego, Calif.)
dc.source.volume31
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1129
dc.identifier.contextkey692121
html.description.abstract<p>Rapid protein purification methodologies, such as strategies involving the tandem affinity purification module, have resulted in the identification of a tremendous number of multisubunit protein complexes. Furthermore, in this modern genomic age, mass spectrometry methods are often coupled with affinity purification to identify the genes that encode each protein subunit. However, simple methodologies to determine the stoichiometry of individual subunits within a multisubunit complex have not received much attention. In this article we describe a procedure to rapidly and efficiently determine the stoichiometry of subunits within multisubunit complexes using a combination of tandem affinity purification and quantitative 125I labeling of subunit tyrosines.</p>
dc.identifier.submissionpathgsbs_sp/1129
dc.contributor.departmentProgram in Gene Function and Expression
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages104-9


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