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    Enhanced Ii expression after n-butyrate treatment of a P3HR-1 Burkitt's lymphoma subline which does not express HLA-D

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    Authors
    Spiro, Robert Christopher
    Sairenji, Takeshi
    Humphreys, Robert E.
    Document Type
    Journal Article
    Publication Date
    1984-07-01
    Keywords
    Burkitt Lymphoma; Butyrates; Butyric Acid; Butyric Acids; Cell Line; Genes, MHC Class II; Histocompatibility Antigens Class II; Humans; Intracellular Membranes; Macromolecular Substances; Membrane Proteins; Microsomes
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://doi.org/10.1002/hon.2900020303
    Abstract
    Treatment of P3HR-1 Burkitt's lymphoma cultured cells with 4 mM n-butyrate for 48 h induced the abundant expression of a 35 000 dalton, microsomal membrane protein which was demonstrated to be the electrophoretically invariant Ii molecule. To prove this point, [35S]methionine-labelled, microsomal membrane proteins or immunoprecipitates of such detergent-solubilized proteins were subjected to electrophoresis either in SDS gels or in two-dimensional, nonequilibrium pH gradient gels. The induced p35 molecule was judged to be Ii by molecular weight and isoelectric characteristics and by immune complex associations. The abundant expression of Ii on butyrate-treated P3HR-1 cells mimicked the high level of expression of p35 or Ii on leukemic cells of a subset of patients with hairy cell leukemia. Alpha and beta chains of the HLA-D complex were absent from the P3HR-1 cell line and present in the Jijoye parent from which mutant P3HR-1 was derived. This relatively simple model can lead to the study of inducible synthesis, processing and expression of Ii, with or without HLA-D alpha and beta chains.
    Source

    Hematol Oncol. 1984 Jul-Sep;2(3):239-47.

    DOI
    10.1002/hon.2900020303
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32587
    PubMed ID
    6434395
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1002/hon.2900020303
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