Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes
AuthorsBoyer, Laurie A.
Becker, Peter B.
Wade, Paul A.
Wolffe, Alan P.
Imbalzano, Anthony N.
Peterson, Craig L.
UMass Chan AffiliationsProgram in Molecular Medicine
Department of Cell Biology
Graduate School of Biomedical Sciences
Document TypeJournal Article
KeywordsAdenosine Triphosphatases; Adenosine Triphosphate; Chromatin; Kinetics; Protein Conformation; Trans-Activators
Medicine and Health Sciences
MetadataShow full item record
AbstractATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We compare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical rates. We also perform assays with arrays reconstituted with hyperacetylated or trypsinized histones and isolated histone (H3/H4)(2) tetramers. The results define three groups of the ATP-dependent family of remodeling enzymes. In addition we investigate the ability of an acidic activator to recruit remodeling complexes to nucleosomal arrays. We propose that ATP-dependent chromatin remodeling enzymes share a common reaction mechanism and that a key distinction between complexes is in their mode of regulation or recruitment.
SourceJ Biol Chem. 2000 Jun 23;275(25):18864-70. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/32628
Related ResourcesLink to article in PubMed