Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes
Authors
Boyer, Laurie A.Logie, Colin
Bonte, Edgar
Becker, Peter B.
Wade, Paul A.
Wolffe, Alan P.
Wu, Carl
Imbalzano, Anthony N.
Peterson, Craig L.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Cell Biology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2000-04-26Keywords
Adenosine Triphosphatases; Adenosine Triphosphate; Chromatin; Kinetics; Protein Conformation; Trans-ActivatorsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
ATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We compare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical rates. We also perform assays with arrays reconstituted with hyperacetylated or trypsinized histones and isolated histone (H3/H4)(2) tetramers. The results define three groups of the ATP-dependent family of remodeling enzymes. In addition we investigate the ability of an acidic activator to recruit remodeling complexes to nucleosomal arrays. We propose that ATP-dependent chromatin remodeling enzymes share a common reaction mechanism and that a key distinction between complexes is in their mode of regulation or recruitment.Source
J Biol Chem. 2000 Jun 23;275(25):18864-70. Link to article on publisher's siteDOI
10.1074/jbc.M002810200Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32628PubMed ID
10779516Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1074/jbc.M002810200