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dc.contributor.authorBoyle, Christine Margaret
dc.contributor.authorRobinson, Harriet L.
dc.date2022-08-11T08:08:49.000
dc.date.accessioned2022-08-23T16:09:20Z
dc.date.available2022-08-23T16:09:20Z
dc.date.issued2000-04-05
dc.date.submitted2008-08-11
dc.identifier.citationDNA Cell Biol. 2000 Mar;19(3):157-65. <a href="http://dx.doi.org/10.1089/104454900314546">Link to article on publisher's site</a>
dc.identifier.issn1044-5498 (Print)
dc.identifier.doi10.1089/104454900314546
dc.identifier.pmid10749168
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32670
dc.description.abstractDNA-raised antibody (Ab) responses have been compared for the dependence on CD4+ and CD8+ cells, the longevity of functional antigen (Ag) expression, and the nature of the Ag-presenting cell after intramuscular (i.m.) and gene gun inoculations. A plasmid expressing the hemagglutinin (HA) glycoprotein of influenza virus was used for immunizations of BALB/c mice. Intramuscular and gene gun-raised Abs had similar dependencies on CD4+ and CD8+ cells but different temporal patterns of functional Ag expression. The two methods of DNA immunization also appeared to have different frequencies or types of Ag-presenting cells in the draining lymph nodes and spleen. For both methods of DNA delivery, Ab was independent of CD8+ cells but dependent on CD4+ cells. The CD4 dependence occurred at priming but not booster immunizations and resulted in a 1-month delay in the Ab response. Temporal T-cell transfers from TCR+/+ mice into immunized TCR-/- mice revealed the persistence of DNA-expressed Ag for up to 1 month after both i.m. and gene gun inoculations. For gene gun, but not i.m. immunizations, approximately 90% of the functional Ag expression was lost by 1 week, consistent with the sloughing of the epidermal target site. Despite similar titers of raised Ab, Ag-presenting dendritic cells could be detected in the draining lymph nodes and spleen of gene gun- but not i.m. DNA-immunized mice. In the gene gun-immunized mice, Ag-presenting dendritic cells appeared in the draining lymph nodes before the spleen.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10749168&dopt=Abstract ">Link to article in PubMed</a>
dc.subjectAnimals; Antibodies, Viral; Antibody Formation; Antibody Specificity; *Biolistics; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Enzyme-Linked Immunosorbent Assay; Female; Hemagglutinin Glycoproteins, Influenza Virus; Injections, Intramuscular; Interferon Type II; Lymphocyte Depletion; Mice; Mice, Inbred BALB C; Mice, Knockout; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocytes; Time Factors; Vaccines, DNA
dc.subjectImmunology and Infectious Disease
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleBasic mechanisms of DNA-raised antibody responses to intramuscular and gene gun immunizations
dc.typeJournal Article
dc.source.journaltitleDNA and cell biology
dc.source.volume19
dc.source.issue3
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1122&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/123
dc.identifier.contextkey573949
refterms.dateFOA2022-08-23T16:09:21Z
html.description.abstract<p>DNA-raised antibody (Ab) responses have been compared for the dependence on CD4+ and CD8+ cells, the longevity of functional antigen (Ag) expression, and the nature of the Ag-presenting cell after intramuscular (i.m.) and gene gun inoculations. A plasmid expressing the hemagglutinin (HA) glycoprotein of influenza virus was used for immunizations of BALB/c mice. Intramuscular and gene gun-raised Abs had similar dependencies on CD4+ and CD8+ cells but different temporal patterns of functional Ag expression. The two methods of DNA immunization also appeared to have different frequencies or types of Ag-presenting cells in the draining lymph nodes and spleen. For both methods of DNA delivery, Ab was independent of CD8+ cells but dependent on CD4+ cells. The CD4 dependence occurred at priming but not booster immunizations and resulted in a 1-month delay in the Ab response. Temporal T-cell transfers from TCR+/+ mice into immunized TCR-/- mice revealed the persistence of DNA-expressed Ag for up to 1 month after both i.m. and gene gun inoculations. For gene gun, but not i.m. immunizations, approximately 90% of the functional Ag expression was lost by 1 week, consistent with the sloughing of the epidermal target site. Despite similar titers of raised Ab, Ag-presenting dendritic cells could be detected in the draining lymph nodes and spleen of gene gun- but not i.m. DNA-immunized mice. In the gene gun-immunized mice, Ag-presenting dendritic cells appeared in the draining lymph nodes before the spleen.</p>
dc.identifier.submissionpathgsbs_sp/123
dc.contributor.departmentDepartment of Pathology
dc.source.pages157-65
dc.contributor.studentChristine M. Boyle


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