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    RISC assembly defects in the Drosophila RNAi mutant armitage

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    Authors
    Tomari, Yukihide
    Du, Tingting
    Haley, Benjamin
    Schwarz, Dianne S.
    Bennett, Ryan
    Cook, Heather A.
    Koppetsch, Birgit S.
    Theurkauf, William E.
    Zamore, Phillip D.
    UMass Chan Affiliations
    Program in Molecular Medicine
    Department of Biochemistry and Molecular Pharmacology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2004-03-24
    Keywords
    Animals; Body Patterning; Cell Differentiation; Drosophila Proteins; Drosophila melanogaster; Embryo, Nonmammalian; Female; Germ Cells; Insect Proteins; Male; Mutation; Oocytes; Protein Kinases; RNA Helicases; RNA Interference; RNA, Small Interfering; RNA-Induced Silencing Complex
    Life Sciences
    Medicine and Health Sciences
    
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    https://doi.org/10.1016/S0092-8674(04)00218-1
    Abstract
    The putative RNA helicase, Armitage (Armi), is required to repress oskar translation in Drosophila oocytes; armi mutant females are sterile and armi mutations disrupt anteroposterior and dorsoventral patterning. Here, we show that armi is required for RNAi. armi mutant male germ cells fail to silence Stellate, a gene regulated endogenously by RNAi, and lysates from armi mutant ovaries are defective for RNAi in vitro. Native gel analysis of protein-siRNA complexes in wild-type and armi mutant ovary lysates suggests that armi mutants support early steps in the RNAi pathway but are defective in the production of active RNA-induced silencing complex (RISC), which mediates target RNA destruction in RNAi. Our results suggest that armi is required for RISC maturation.
    Source

    Cell. 2004 Mar 19;116(6):831-41.

    DOI
    10.1016/S0092-8674(04)00218-1
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32698
    PubMed ID
    15035985
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1016/S0092-8674(04)00218-1
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