Translational unmasking of Emi2 directs cytostatic factor arrest in meiosis II
UMass Chan Affiliations
Program in Molecular MedicineGenentech
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2007-03-16Keywords
Animals; F-Box Proteins; Female; Meiosis; Oocytes; Protein Biosynthesis; Proto-Oncogene Proteins c-mos; Rabbits; Xenopus; Xenopus ProteinsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Cytostatic factor (CSF) arrests unfertilized vertebrate eggs in metaphase of meiosis II by inhibiting the anaphase-promoting complex/cyclosome (APC/C) from mediating cyclin destruction. The APC/C inhibitor Emi2/XErp1 satisfies a number of historical criteria for the molecular identification of CSF, but the mechanism by which CSF is activated selectively in meiosis II is the remaining unexplained criterion. Here we provide an explanation by showing that Emi2 is expressed specifically in meiosis II through translational de-repression or "unmasking" of its mRNA. We find that Emi2 protein is undetectable in immature, G2/prophase-arrested Xenopus oocytes and accumulates approximately 90 minutes after germinal vesicle breakdown. The 3' untranslated region of Emi2 mRNA contains cytoplasmic polyadenylation elements that directly bind the CPEB protein and confer temporal regulation of Emi2 polyadenylation and translation. Our results demonstrate that cytoplasmic polyadenylation and translational unmasking of Emi2 directs meiosis II-specific CSF arrest.Source
Cell Cycle. 2007 Mar 15;6(6):725-31. Epub 2007 Mar 1.
DOI
10.4161/cc.6.6.3936Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32709PubMed ID
17361107Related Resources
ae974a485f413a2113503eed53cd6c53
10.4161/cc.6.6.3936