Detection of a high frequency of virus-specific CD4+ T cells during acute infection with lymphocytic choriomeningitis virus
| dc.contributor.author | Varga, Steven Michael | |
| dc.contributor.author | Welsh, Raymond M. | |
| dc.date | 2022-08-11T08:08:50.000 | |
| dc.date.accessioned | 2022-08-23T16:09:39Z | |
| dc.date.available | 2022-08-23T16:09:39Z | |
| dc.date.issued | 1998-10-06 | |
| dc.date.submitted | 2009-01-13 | |
| dc.identifier.citation | <p>J Immunol. 1998 Oct 1;161(7):3215-8.</p> | |
| dc.identifier.issn | 0022-1767 (Print) | |
| dc.identifier.pmid | 9759834 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/32748 | |
| dc.description.abstract | Lymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9759834&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | http://www.jimmunol.org/content/161/7/3215.long | |
| dc.subject | Acute Disease; Animals; CD4-Positive T-Lymphocytes; Epitopes, T-Lymphocyte; Interferon Type II; Intracellular Fluid; Lymphocyte Count; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Male; Mice; Mice, Inbred C57BL; Peptides; Staining and Labeling; Stem Cells; Viral Proteins | |
| dc.subject | Life Sciences | |
| dc.subject | Medicine and Health Sciences | |
| dc.title | Detection of a high frequency of virus-specific CD4+ T cells during acute infection with lymphocytic choriomeningitis virus | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Journal of immunology (Baltimore, Md. : 1950) | |
| dc.source.volume | 161 | |
| dc.source.issue | 7 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/1301 | |
| dc.identifier.contextkey | 693230 | |
| html.description.abstract | <p>Lymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections.</p> | |
| dc.identifier.submissionpath | gsbs_sp/1301 | |
| dc.contributor.department | Department of Pathology | |
| dc.contributor.department | Graduate School of Biomedical Sciences | |
| dc.source.pages | 3215-8 |
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