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dc.contributor.authorVarga, Steven Michael
dc.contributor.authorWelsh, Raymond M.
dc.date2022-08-11T08:08:50.000
dc.date.accessioned2022-08-23T16:09:39Z
dc.date.available2022-08-23T16:09:39Z
dc.date.issued1998-10-06
dc.date.submitted2009-01-13
dc.identifier.citation<p>J Immunol. 1998 Oct 1;161(7):3215-8.</p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.pmid9759834
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32748
dc.description.abstractLymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9759834&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttp://www.jimmunol.org/content/161/7/3215.long
dc.subjectAcute Disease; Animals; CD4-Positive T-Lymphocytes; Epitopes, T-Lymphocyte; Interferon Type II; Intracellular Fluid; Lymphocyte Count; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Male; Mice; Mice, Inbred C57BL; Peptides; Staining and Labeling; Stem Cells; Viral Proteins
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleDetection of a high frequency of virus-specific CD4+ T cells during acute infection with lymphocytic choriomeningitis virus
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume161
dc.source.issue7
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1301
dc.identifier.contextkey693230
html.description.abstract<p>Lymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections.</p>
dc.identifier.submissionpathgsbs_sp/1301
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages3215-8


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