UMass Chan Affiliations
Program in Molecular MedicineHoward Hughes Medical Institute
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1996-09-12Keywords
*Antigens, Neoplasm; Carrier Proteins; DNA-Binding Proteins; Gene Expression Regulation, Fungal; Heat-Shock Response; *Histocompatibility Antigens; Metallothionein; Molecular Sequence Data; Mutation; Saccharomyces cerevisiae; TATA Box; TATA-Box Binding Protein; Temperature; *Trans-Activation (Genetics); Transcription Factor TFIID; Transcription Factors; Transcription, GeneticLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The general transcription factor TFIID is composed of the TATA-box-binding protein (TBP) and a set of TBP-associated factors (TAFIIs). In vitro, TAFIIs are required for activated transcription, and have been proposed to be obligatory targets of transcriptional activator proteins (activators)2. The function of TAFIIs has not been investigated systematically in vivo. A Saccharomyces cerevisiae TAFII complex (yTAFII complex) has been identified that shares functional and structural similarities with higher eukaryotic TFIID. In particular, most yTAFIIs are the homologue of a higher eukaryotic TAFII. Here we report that inactivation or depletion of six different yTAFIIs, including the core yTAFII, that contacts TBP, does not compromise transcriptional activation. We conclude that in vivo, activated transcription of many genes can occur in the absence of functional yTAFIIS, and that in these instances another transcription component(s) must be the target of the activator.Source
Nature. 1996 Sep 12;383(6596):185-8. Link to article on publisher's siteDOI
10.1038/383185a0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32770PubMed ID
8774886Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/383185a0