UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2005-04-27Keywords
Base Sequence; Binding Sites; Catalysis; Cyclin-Dependent Kinase 9; HIV Long Terminal Repeat; Hela Cells; Humans; Kinetics; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Viral; RNA-Induced Silencing ComplexLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.Source
Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's siteDOI
10.1038/nsmb931Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32776PubMed ID
15852021Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/nsmb931