Crystallization and preliminary X-ray analysis of a domain in the Runx2 transcription factor that interacts with the 1alpha,25 dihydroxy vitamin D3 receptor
Authors
Bruna, CarolaArriagada, Gloria
Lian, Jane B.
Stein, Gary S.
Bunster, Marta
Martinez-Oyanedel, Jose
Montecino, Martin A.
Document Type
Journal ArticlePublication Date
2007-01-18Keywords
Absorptiometry, Photon; Animals; Core Binding Factor Alpha 1 Subunit; Crystallization; Mice; Protein Binding; Protein Structure, Tertiary; Receptors, CalcitriolLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The Runx2 transcription factor is a key regulator of osteoblast differentiation. In response to 1alpha,25 dihydroxy vitamin D3, Runx2 may interact with the 1alpha,25 dihydroxy vitamin D3 receptor (VDR) in the promoter of target genes, producing a synergic activation of their transcription. Previous studies have suggested that the motifs responsible for the VDR-Runx2 interaction are contained within the 230-361 domain of Runx2. In this work, we confirmed by GST-pull down that Runx2(I(209-361)) is sufficient to interact with the VDR. To obtain structural information, GST-Runx2(I(209-361)) protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapor-diffusion method and polyethyleneglycol as a precipitant. The crystals were found to diffract to a maximum resolution of 2.7 A and a complete data set to a 3.3 A resolution was collected and analyzed. The crystals belong to the tetragonal system, with a space group P4 and unit-cell parameters of a = b = 90.8, and c = 57.2 A. The presence of a monomer of the recombinant GST-Runx2(I(209-361)) in the asymmetric unit gives a V(M) of 2.7 A(3) Da(-1) and a solvent content of 54.8%.Source
J Cell Biochem. 2007 Jun 1;101(3):785-9. Link to article on publisher's siteDOI
10.1002/jcb.21231Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32797PubMed ID
17226779Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcb.21231