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    Immunogenicity and protection efficacy of subunit-based smallpox vaccines using variola major antigens

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    Authors
    Sakhatskyy, Pavlo V.
    Wang, Shixia
    Zhang, Chuanyou
    Chou, Te-Hui
    Kishko, Michael G.
    Lu, Shan
    Student Authors
    Michael G. Kishko
    UMass Chan Affiliations
    Department of Medicine, Division of Infectious Diseases and Immunology
    Document Type
    Journal Article
    Publication Date
    2008-02-05
    Keywords
    Amino Acid Sequence; Animals; Antigens, Viral; Body Weight; Cell Line; Chemoprevention; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Feasibility Studies; Female; Humans; Immunization Schedule; Immunoglobulin G; Kidney; Mice; Mice, Inbred BALB C; Models, Animal; Molecular Sequence Data; Neutralization Tests; Sequence Homology, Amino Acid; Smallpox; Smallpox Vaccine; Vaccines, Attenuated; Vaccines, DNA; Vaccines, Subunit; Vaccinia virus; Variola virus; *Viral Vaccines
    Immunology and Infectious Disease
    Life Sciences
    Medicine and Health Sciences
    Microbiology
    
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    http://dx.doi.org/10.1016/j.virol.2007.09.029
    Abstract
    The viral strain responsible for smallpox infection is variola major (VARV). As a result of the successful eradication of smallpox with the vaccinia virus (VACV), the general population is no longer required to receive a smallpox vaccine, and will have no protection against smallpox. This lack of immunity is a concern due to the potential for use of smallpox as a biological weapon. Considerable progress has been made in the development of subunit-based smallpox vaccines resulting from the identification of VACV protective antigens. It also offers the possibility of using antigens from VARV to formulate the next generation subunit-based smallpox vaccines. Here, we show that codon-optimized DNA vaccines expressing three VARV antigens (A30, B7 and F8) and their recombinant protein counterparts elicited high-titer, cross-reactive, VACV neutralizing antibody responses in mice. Vaccinated mice were protected from intraperitoneal and intranasal challenges with VACV. These results suggest the feasibility of a subunit smallpox vaccine based on VARV antigen sequences to induce immunity against poxvirus infection.
    Source
    Virology. 2008 Feb 5;371(1):98-107. Epub 2007 Oct 24. Link to article on publisher's site
    DOI
    10.1016/j.virol.2007.09.029
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32804
    PubMed ID
    17950773
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    ae974a485f413a2113503eed53cd6c53
    10.1016/j.virol.2007.09.029
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