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dc.contributor.authorBrundage, Rodney Arthur
dc.contributor.authorFogarty, Kevin E.
dc.contributor.authorTuft, Richard A.
dc.contributor.authorFay, Fredric S.
dc.date2022-08-11T08:08:51.000
dc.date.accessioned2022-08-23T16:09:55Z
dc.date.available2022-08-23T16:09:55Z
dc.date.issued1993-12-01
dc.date.submitted2008-08-11
dc.identifier.citation<p>Am J Physiol. 1993 Dec;265(6 Pt 1):C1527-43.</p>
dc.identifier.issn0002-9513 (Print)
dc.identifier.doi10.1152/ajpcell.1993.265.6.C1527
dc.identifier.pmid8279515
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32815
dc.description.abstractLocal chemical events underlying chemotaxis were characterized in a new model cell, the newt eosinophil. These cells exhibit a chemotactic response to a trypsin-sensitive component of newt serum. Ca2+ plays a role in this process, since treatments expected to diminish Ca2+ availability from the medium [ethylene glycol-bis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, Co2+, and verapamil], to break down transmembrane Ca2+ gradients (ionomycin), or to interfere with the function of intracellular Ca2+ stores (caffeine and neomycin) inhibited cell polarization and movement. Using imaging techniques we found that cytosolic Ca2+ concentration ([Ca2+]i) increased in response to newt serum. Migrating newt eosinophils exhibited a dynamic heterogeneous distribution of [Ca2+]i. [Ca2+]i was elevated in cells undergoing a change of direction relative to cells migrating persistently in one direction. Migrating cells contained gradients of [Ca2+]i along their long axis, with the front of the cell having consistently lower [Ca2+]i than the rear. When cells were loaded with the cell-permeant form of fura 2, fura 2 acetoxymethyl ester, a caffeine-sensitive membrane-delimited region of elevated [Ca2+]i was seen associated with the microtubule organizing center. A model is proposed relating the distribution of [Ca2+]i and the location of the external stimulus to the generation and interaction of substances within the cell that both simulate and inhibit increases in [Ca2+]i.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8279515&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1152/ajpcell.1993.265.6.C1527
dc.subjectAmino Acid Sequence; Animals; Caffeine; Calcium; Chemotactic Factors, Eosinophil; Chemotaxis, Leukocyte; Chromatography, Gel; Cobalt; Egtazic Acid; Eosinophils; Fluorescent Dyes; Fura-2; Ionomycin; Molecular Sequence Data; Neomycin; Salamandridae; Verapamil
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleChemotaxis of newt eosinophils: calcium regulation of chemotactic response
dc.typeJournal Article
dc.source.journaltitleThe American journal of physiology
dc.source.volume265
dc.source.issue6 Pt 1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/137
dc.identifier.contextkey573963
html.description.abstract<p>Local chemical events underlying chemotaxis were characterized in a new model cell, the newt eosinophil. These cells exhibit a chemotactic response to a trypsin-sensitive component of newt serum. Ca2+ plays a role in this process, since treatments expected to diminish Ca2+ availability from the medium [ethylene glycol-bis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, Co2+, and verapamil], to break down transmembrane Ca2+ gradients (ionomycin), or to interfere with the function of intracellular Ca2+ stores (caffeine and neomycin) inhibited cell polarization and movement. Using imaging techniques we found that cytosolic Ca2+ concentration ([Ca2+]i) increased in response to newt serum. Migrating newt eosinophils exhibited a dynamic heterogeneous distribution of [Ca2+]i. [Ca2+]i was elevated in cells undergoing a change of direction relative to cells migrating persistently in one direction. Migrating cells contained gradients of [Ca2+]i along their long axis, with the front of the cell having consistently lower [Ca2+]i than the rear. When cells were loaded with the cell-permeant form of fura 2, fura 2 acetoxymethyl ester, a caffeine-sensitive membrane-delimited region of elevated [Ca2+]i was seen associated with the microtubule organizing center. A model is proposed relating the distribution of [Ca2+]i and the location of the external stimulus to the generation and interaction of substances within the cell that both simulate and inhibit increases in [Ca2+]i.</p>
dc.identifier.submissionpathgsbs_sp/137
dc.contributor.departmentDepartment of Physiology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pagesC1527-43


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