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dc.contributor.authorSun, Xiangao
dc.contributor.authorRogoff, Harry A.
dc.contributor.authorLi, Chiang J.
dc.date2022-08-11T08:08:51.000
dc.date.accessioned2022-08-23T16:09:56Z
dc.date.available2022-08-23T16:09:56Z
dc.date.issued2008-11-26
dc.date.submitted2009-02-19
dc.identifier.citationNat Biotechnol. 2008 Dec;26(12):1379-82. Epub 2008 Nov 23. <a href="http://dx.doi.org/10.1038/nbt.1512">Link to article on publisher's site</a>
dc.identifier.issn1546-1696 (Electronic)
dc.identifier.doi10.1038/nbt.1512
dc.identifier.pmid19029911
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32820
dc.description.abstractRNA interference (RNAi) has become an indispensable technology for biomedical research and has demonstrated the potential to become a new class of therapeutic. Current RNAi technology in mammalian cells relies on short interfering RNA (siRNA) consisting of symmetrical duplexes of 19-21 base pairs (bp) with 3' overhangs. Here we report that asymmetric RNA duplexes with 3' and 5' antisense overhangs silence mammalian genes effectively. An asymmetric interfering RNA (aiRNA) of 15 bp was incorporated into the RNA-induced silencing complex (RISC) and mediated sequence-specific cleavage of the target mRNA between base 10 and 11 relative to the 5' end of the antisense strand. The gene silencing mediated by aiRNA was efficacious, durable and correlated with reduced off-target silencing by the sense strand. These results establish aiRNA as a scaffold structure for designing RNA duplexes to induce RNAi in mammalian cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19029911&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1038/nbt.1512
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleAsymmetric RNA duplexes mediate RNA interference in mammalian cells
dc.typeJournal Article
dc.source.journaltitleNature biotechnology
dc.source.volume26
dc.source.issue12
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1376
dc.identifier.contextkey727572
html.description.abstract<p>RNA interference (RNAi) has become an indispensable technology for biomedical research and has demonstrated the potential to become a new class of therapeutic. Current RNAi technology in mammalian cells relies on short interfering RNA (siRNA) consisting of symmetrical duplexes of 19-21 base pairs (bp) with 3' overhangs. Here we report that asymmetric RNA duplexes with 3' and 5' antisense overhangs silence mammalian genes effectively. An asymmetric interfering RNA (aiRNA) of 15 bp was incorporated into the RNA-induced silencing complex (RISC) and mediated sequence-specific cleavage of the target mRNA between base 10 and 11 relative to the 5' end of the antisense strand. The gene silencing mediated by aiRNA was efficacious, durable and correlated with reduced off-target silencing by the sense strand. These results establish aiRNA as a scaffold structure for designing RNA duplexes to induce RNAi in mammalian cells.</p>
dc.identifier.submissionpathgsbs_sp/1376
dc.contributor.departmentDepartment of Therapeutic Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages1379-82


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