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dc.contributor.authorSanchez-Merino, Victor
dc.contributor.authorFarrow, Melissa Ann
dc.contributor.authorFarrow, Melissa Ann
dc.contributor.authorBrewster, Frank E.
dc.contributor.authorSomasundaran, Mohan
dc.contributor.authorLuzuriaga, Katherine
dc.contributor.authorBrewster, Frank E.
dc.contributor.authorSomasundaran, Mohan
dc.contributor.authorLuzuriaga, Katherine
dc.date2022-08-11T08:08:51.000
dc.date.accessioned2022-08-23T16:10:11Z
dc.date.available2022-08-23T16:10:11Z
dc.date.issued2008-01-08
dc.date.submitted2009-02-19
dc.identifier.citationJ Infect Dis. 2008 Jan 15;197(2):300-8. <a href="http://dx.doi.org/10.1086/524845">Link to article on publisher's site</a>
dc.identifier.issn0022-1899 (Print)
dc.identifier.doi10.1086/524845
dc.identifier.pmid18177249
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32875
dc.description.abstractIn this study, amino acid sequence variation in human immunodeficiency virus (HIV)-1 Gag CD8(+) T cell epitopes was examined in untreated mother-infant pairs. Several HIV-1 CD8(+) T cell escape variants were identified within maternal plasma viral p17 and p24 sequences that were either not detected or did not persist in the plasma of their non-HLA-matched HIV-1-infected infants. Viruses constructed with each of these mutations demonstrated reduced viral replication in vitro and reduced expression of p17 and p24 proteins compared with wild type. Reduced recognition of the variant sequences compared with wild-type sequence was also demonstrated by enzyme-linked immunospot assays. Nontransmission or reversion after transmission was thus associated with reduced viral fitness cost in vivo. Better understanding of the balance between CD8(+) T cell selective pressures and viral fitness cost may facilitate the identification of optimal viral sequences for inclusion in HIV-1 vaccines.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18177249&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1086/524845
dc.subjectAmino Acid Sequence; Amino Acid Substitution; CD8-Positive T-Lymphocytes; Epitopes, T-Lymphocyte; Female; HIV Antigens; HIV Core Protein p24; HIV Infections; HIV-1; Hela Cells; Humans; Infant; Infectious Disease Transmission, Vertical; Molecular Sequence Data; *Mutation; Sequence Analysis, DNA; Transfection; Virus Replication; gag Gene Products, Human Immunodeficiency; Virus
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleIdentification and characterization of HIV-1 CD8+ T cell escape variants with impaired fitness
dc.typeJournal Article
dc.source.journaltitleThe Journal of infectious diseases
dc.source.volume197
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1428
dc.identifier.contextkey727720
html.description.abstract<p>In this study, amino acid sequence variation in human immunodeficiency virus (HIV)-1 Gag CD8(+) T cell epitopes was examined in untreated mother-infant pairs. Several HIV-1 CD8(+) T cell escape variants were identified within maternal plasma viral p17 and p24 sequences that were either not detected or did not persist in the plasma of their non-HLA-matched HIV-1-infected infants. Viruses constructed with each of these mutations demonstrated reduced viral replication in vitro and reduced expression of p17 and p24 proteins compared with wild type. Reduced recognition of the variant sequences compared with wild-type sequence was also demonstrated by enzyme-linked immunospot assays. Nontransmission or reversion after transmission was thus associated with reduced viral fitness cost in vivo. Better understanding of the balance between CD8(+) T cell selective pressures and viral fitness cost may facilitate the identification of optimal viral sequences for inclusion in HIV-1 vaccines.</p>
dc.identifier.submissionpathgsbs_sp/1428
dc.contributor.departmentInterdisciplinary Graduate Program
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages300-8


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