• Login
    View Item 
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside GSBS Scholarly Publications
    • View Item
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside GSBS Scholarly Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsProfilesView

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingUsage StatisticsAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    zaidi_structural.pdf
    Size:
    778.2Kb
    Format:
    PDF
    Download
    Authors
    Javed, Amjad
    Bae, Jong-Sup
    Afzal, Faiza
    Gutierrez, Soraya E.
    Pratap, Jitesh
    Zaidi, Sayyed K.
    Lou, Yang
    Van Wijnen, Andre J.
    Stein, Janet L.
    Stein, Gary S.
    Lian, Jane B.
    Show allShow less
    UMass Chan Affiliations
    Department of Cell Biology and Cancer Center
    Document Type
    Journal Article
    Publication Date
    2008-01-22
    Keywords
    Amino Acid Sequence; Animals; Biological Markers; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Cell Differentiation; Core Binding Factor Alpha 1; Subunit; Hela Cells; Humans; Mice; Mice, Knockout; Molecular Sequence Data; Mutation; Osteoblasts; *Osteogenesis; Phenotype; Protein Binding; *Signal Transduction; Smad Proteins; Transforming Growth Factor beta
    Life Sciences
    Medicine and Health Sciences
    
    Metadata
    Show full item record
    Abstract
    Two regulatory pathways, bone morphogenetic protein (BMP)/transforming growth factor-beta (TGFbeta) and the transcription factor RUNX2, are required for bone formation in vivo. Here we show the interdependent requirement of these pathways to induce an osteogenic program. A panel of Runx2 deletion and point mutants was used to examine RUNX2-SMAD protein-protein interaction and the biological consequences on BMP2-induced osteogenic signaling determined in Runx2 null cells. These cells do not respond to BMP2 signal in the absence of Runx2. We established that a triple mutation in the C-terminal domain of RUNX2, HTY (426-428), disrupts the RUNX2-SMAD interaction, is deficient in its ability to integrate the BMP2/TGFbeta signal on promoter reporter assays, and is only marginally functional in promoting early stages of osteoblast differentiation. Furthermore, the HTY mutation overlaps the unique nuclear matrix targeting signal of Runx factors and exhibits reduced subnuclear targeting. Thus, formation of a RUNX2-SMAD osteogenic complex and subnuclear targeting are structurally and functionally inseparable. Our results establish the critical residues of RUNX2 for execution and completion of BMP2 signaling for osteoblastogenesis through a mechanism that requires RUNX2-SMAD transcriptional activity.
    Source
    J Biol Chem. 2008 Mar 28;283(13):8412-22. Epub 2008 Jan 18. Link to article on publisher's site
    DOI
    10.1074/jbc.M705578200
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32879
    PubMed ID
    18204048
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1074/jbc.M705578200
    Scopus Count
    Collections
    Morningside GSBS Scholarly Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Works found in eScholarship@UMassChan are protected by copyright unless otherwise indicated.
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.