Show simple item record

dc.contributor.authorTowne, Charles Fenton
dc.contributor.authorYork, Ian A.
dc.contributor.authorNeijssen, Joost J.
dc.contributor.authorKarow, Margaret L.
dc.contributor.authorMurphy, Andrew J.
dc.contributor.authorValenzuela, David M.
dc.contributor.authorYancopoulos, George D.
dc.contributor.authorNeefjes, Jacques J.
dc.contributor.authorRock, Kenneth L.
dc.date2022-08-11T08:08:51.000
dc.date.accessioned2022-08-23T16:10:13Z
dc.date.available2022-08-23T16:10:13Z
dc.date.issued2008-01-23
dc.date.submitted2009-02-19
dc.identifier.citation<p>J Immunol. 2008 Feb 1;180(3):1704-12.</p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.doi10.4049/jimmunol.180.3.1704
dc.identifier.pmid18209067
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32880
dc.description.abstractPrevious experiments using enzyme inhibitors, cell lysates, and purified enzyme have suggested that puromycin-sensitive aminopeptidase (PSA) plays a role in creating and destroying MHC class I-presented peptides although its precise contribution to these processes is unknown. To examine the importance of this enzyme in MHC class I Ag presentation, we have generated PSA-deficient mice and cell lines from these animals. PSA-deficient mice are smaller and do not reproduce as well as wild type mice. In addition, dendritic cells from PSA-deficient mice display more MHC class I molecules on the cell surface, suggesting that PSA normally limits Ag presentation by destroying certain peptides in these key APCs. Surprisingly, MHC class I levels are not altered on other PSA-deficient cells and the processing and presentation of peptide precursors in PSA-deficient fibroblasts is normal. Moreover, PSA-deficient mice have normal numbers of T cells in the periphery, and respond as well as wild type mice to eight epitopes from three viruses. These data indicate that PSA may play a role in limiting MHC class I Ag presentation in dendritic cells in vivo but that it is not essential for generating most MHC class I-presented peptides or for stimulating CTL responses to several Ags.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18209067&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.4049/jimmunol.180.3.1704
dc.subjectAmino Acid Sequence; Aminopeptidases; Animals; *Antigen Presentation; Antigens, Viral; CD8-Positive T-Lymphocytes; Dendritic Cells; Epitopes; Histocompatibility Antigens Class I; Mice; Mice, Mutant Strains; Molecular Sequence Data; Peptides; Virus Diseases; Viruses
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titlePuromycin-sensitive aminopeptidase limits MHC class I presentation in dendritic cells but does not affect CD8 T cell responses during viral infections
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume180
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1433
dc.identifier.contextkey727727
html.description.abstract<p>Previous experiments using enzyme inhibitors, cell lysates, and purified enzyme have suggested that puromycin-sensitive aminopeptidase (PSA) plays a role in creating and destroying MHC class I-presented peptides although its precise contribution to these processes is unknown. To examine the importance of this enzyme in MHC class I Ag presentation, we have generated PSA-deficient mice and cell lines from these animals. PSA-deficient mice are smaller and do not reproduce as well as wild type mice. In addition, dendritic cells from PSA-deficient mice display more MHC class I molecules on the cell surface, suggesting that PSA normally limits Ag presentation by destroying certain peptides in these key APCs. Surprisingly, MHC class I levels are not altered on other PSA-deficient cells and the processing and presentation of peptide precursors in PSA-deficient fibroblasts is normal. Moreover, PSA-deficient mice have normal numbers of T cells in the periphery, and respond as well as wild type mice to eight epitopes from three viruses. These data indicate that PSA may play a role in limiting MHC class I Ag presentation in dendritic cells in vivo but that it is not essential for generating most MHC class I-presented peptides or for stimulating CTL responses to several Ags.</p>
dc.identifier.submissionpathgsbs_sp/1433
dc.contributor.departmentDepartment of Pathology
dc.source.pages1704-12


This item appears in the following Collection(s)

Show simple item record