Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay
AuthorsDzutsev, Amiran K.
Belyakov, Igor M.
Isakov, Dmitry V.
Gagnon, Susan J.
Margulies, David H.
Berzofsky, Jay A.
Document TypeJournal Article
KeywordsAnimals; Antigen Presentation; Antigen-Presenting Cells; Antigens, Viral; CD8-Positive T-Lymphocytes; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte; Female; Flow Cytometry; Fluorescent Dyes; Interferon-gamma; Mice; Mice, Inbred BALB C; Microscopy, Fluorescence; Ovary; Rosette Formation; Vaccinia; Vaccinia virus
Medicine and Health Sciences
MetadataShow full item record
AbstractAdequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge.
SourceJ Immunol Methods. 2008 Apr 20;333(1-2):71-8. Epub 2008 Feb 8. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/32906
Related ResourcesLink to Article in PubMed