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dc.contributor.authorYang, Kejian
dc.contributor.authorWhalen, Barbara J.
dc.contributor.authorTirabassi, Rebecca S
dc.contributor.authorSelin, Liisa K.
dc.contributor.authorLevchenko, Tatyana S.
dc.contributor.authorTorchilin, Vladimir P.
dc.contributor.authorKislauskis, Edward H.
dc.contributor.authorGuberski, Dennis L.
dc.date2022-08-11T08:08:52.000
dc.date.accessioned2022-08-23T16:10:27Z
dc.date.available2022-08-23T16:10:27Z
dc.date.issued2008-04-22
dc.date.submitted2009-02-24
dc.identifier.citation<p>J Immunol. 2008 May 1;180(9):6159-67.</p>
dc.identifier.issn0022-1767 (Print)
dc.identifier.doi10.4049/jimmunol.180.9.6159
dc.identifier.pmid18424737
dc.identifier.urihttp://hdl.handle.net/20.500.14038/32935
dc.description.abstractA variety of DNA vaccine prime and recombinant viral boost immunization strategies have been developed to enhance immune responses in humans, but inherent limitations to these strategies exist. There is still an overwhelming need to develop safe and effective approaches that raise broad humoral and T cell-mediated immune responses systemically and on mucosal surfaces. We have developed a novel mucosal immunization regimen that precludes the use of viral vectors yet induces potent T cell responses. Using hepatitis B surface Ag (HBsAg), we observed that vaccination of BALB/c mice with an i.m. HBsAg-DNA vaccine prime followed by an intranasal boost with HBsAg protein encapsulated in biologically inert liposomes enhanced humoral and T cell immune responses, particularly on mucosal surfaces. Intranasal live virus challenge with a recombinant vaccinia virus expressing HBsAg revealed a correlation between T cell immune responses and protection of immunized mice. A shortened immunization protocol was developed that was successful in both adult and neonatal mice. These results support the conclusion that this new approach is capable of generating a Th-type-1-biased, broad spectrum immune response, specifically at mucosal surfaces. The success of this design may provide a safe and effective vaccination alternative for human use.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18424737&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633597/
dc.subjectAdministration, Intranasal; Animals; Animals, Newborn; Antibody Formation; Female; Hepatitis B Surface Antigens; Hepatitis B Vaccines; Humans; Immunity, Mucosal; *Immunization, Secondary; Liposomes; Mice; Mice, Inbred BALB C; Th1 Cells; Vaccines, DNA; Vaccinia virus
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA DNA vaccine prime followed by a liposome-encapsulated protein boost confers enhanced mucosal immune responses and protection
dc.typeJournal Article
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume180
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1487
dc.identifier.contextkey738052
html.description.abstract<p>A variety of DNA vaccine prime and recombinant viral boost immunization strategies have been developed to enhance immune responses in humans, but inherent limitations to these strategies exist. There is still an overwhelming need to develop safe and effective approaches that raise broad humoral and T cell-mediated immune responses systemically and on mucosal surfaces. We have developed a novel mucosal immunization regimen that precludes the use of viral vectors yet induces potent T cell responses. Using hepatitis B surface Ag (HBsAg), we observed that vaccination of BALB/c mice with an i.m. HBsAg-DNA vaccine prime followed by an intranasal boost with HBsAg protein encapsulated in biologically inert liposomes enhanced humoral and T cell immune responses, particularly on mucosal surfaces. Intranasal live virus challenge with a recombinant vaccinia virus expressing HBsAg revealed a correlation between T cell immune responses and protection of immunized mice. A shortened immunization protocol was developed that was successful in both adult and neonatal mice. These results support the conclusion that this new approach is capable of generating a Th-type-1-biased, broad spectrum immune response, specifically at mucosal surfaces. The success of this design may provide a safe and effective vaccination alternative for human use.</p>
dc.identifier.submissionpathgsbs_sp/1487
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentOral Vaccine Institute
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages6159-67


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