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    IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation

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    Authors
    Mortier, Erwan
    Woo, Tammy
    Advincula, Rommel
    Gozalo, Sara
    Ma, Averil
    UMass Chan Affiliations
    Colitis and Crohn's Disease Center
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2008-05-07
    Keywords
    Adoptive Transfer; Animals; Cell Communication; Cell Membrane; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Interleukin-15; Interleukin-15 Receptor alpha Subunit; Killer Cells, Natural; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Mice, Knockout
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1084/jem.20071913
    Abstract
    Natural killer (NK) cells are innate immune effectors that mediate rapid responses to viral antigens. Interleukin (IL)-15 and its high affinity IL-15 receptor, IL-15Ralpha, support NK cell homeostasis in resting animals via a novel trans presentation mechanism. To better understand how IL-15 and IL-15Ralpha support NK cell activation during immune responses, we have used sensitive assays for detecting native IL-15 and IL-15Ralpha proteins and developed an assay for detecting complexes of these proteins. We find that IL-15 and IL-15Ralpha are preassembled in complexes within the endoplasmic reticulum/Golgi of stimulated dendritic cells (DCs) before being released from cells. IL-15Ralpha is required for IL-15 production by DCs, and IL-15 that emerges onto the cell surface of matured DCs does not bind to neighboring cells expressing IL-15Ralpha. We also find that soluble IL-15-IL-15Ralpha complexes are induced during inflammation, but membrane-bound IL-15-IL-15Ralpha complexes, rather than soluble complexes, support NK cell activation in vitro and in vivo. Finally, we provide in vivo evidence that expression of IL-15Ralpha specifically on DCs is critical for trans presenting IL-15 and activating NK cells. These studies define an unprecedented cytokine-receptor biosynthetic pathway in which IL-15Ralpha serves as a chaperone for IL-15, after which membrane-bound IL-15Ralpha-IL-15 complexes activate NK cells via direct cell-cell contact.
    Source
    J Exp Med. 2008 May 12;205(5):1213-25. Epub 2008 May 5. Link to article on publisher's site
    DOI
    10.1084/jem.20071913
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/32945
    PubMed ID
    18458113
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1084/jem.20071913
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