Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
AuthorsJuette, Manuel F.
Gould, Travis J.
Lessard, Mark D.
Mlodzianoski, Michael J.
Nagpure, Bhupendra S.
Bennett, Brian Thomas
Hess, Samuel T.
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Graduate School of Biomedical Sciences
KeywordsBiophysics; Fluorescein; Fluorescent Dyes; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Lasers; Light; Microscopy; Microscopy, Fluorescence; Software
Medicine and Health Sciences
MetadataShow full item record
AbstractImaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
SourceNat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/32950
Related ResourcesLink to Article in PubMed