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dc.contributor.authorCable, Edward Earl
dc.contributor.authorGildemeister, Otto S.
dc.contributor.authorPepe, Joyce A.
dc.contributor.authorLambrecht, Richard W.
dc.contributor.authorBonkovsky, Herbert L.
dc.date2022-08-11T08:08:53.000
dc.date.accessioned2022-08-23T16:10:57Z
dc.date.available2022-08-23T16:10:57Z
dc.date.issued1997-04-01
dc.date.submitted2008-08-14
dc.identifier.citation<p>Mol Cell Biochem. 1997 Apr;169(1-2):13-20.</p>
dc.identifier.issn0300-8177 (Print)
dc.identifier.doi10.1023/A:1006817207166
dc.identifier.pmid9089626
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33052
dc.description.abstractHeme oxygenase catalyzes the first and rate-controlling step in heme catabolism. One of the two forms of heme oxygenase (heme oxygenase-1) has been shown to be increased by heme, metals, and in some systems, by certain environmental stresses. However, it remains uncertain whether heme induces hepatic heme oxygenase-1 by a general stress response, or a specific heme-dependent cellular response. The work communicated here explores this issue by examining possible mechanisms whereby heme and other metalloporphyrins induce heme oxygenase-1 in normal liver cells. Primary cultures of chick embryo liver cells were tested for their ability to increase heme oxygenase mRNA after exposure to selected metalloporphyrins (heme, chromium mesoporphyrin, cobalt protoporphyrin and manganese protoporphyrin). The ability of antioxidants to decrease metalloporphyrin-mediated induction of heme oxygenase-1 mRNA was also tested. Our results indicate that: 1) the increase in heme oxygenase-1 mRNA mediated by heme or other metalloporphyrins may involve a short-lived protein(s) since the increase was prevented by several inhibitors of protein synthesis; and 2) in normal liver cells, heme-dependent oxidative stress does not play a key role in the heme-mediated induction of heme oxygenase-1. We conclude that heme and other non-heme metalloporphyrins induce heme oxygenase-1 through a mechanism requiring protein synthesis, not because metalloporphyrins increase cellular oxidative or other stress.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9089626&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1023/A:1006817207166
dc.subjectAnimals; Antioxidants; Chick Embryo; Gene Expression Regulation; Heme; Heme Oxygenase (Decyclizing); Lipid Peroxidation; Liver; Metalloporphyrins; RNA, Messenger
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleMechanism of induction of heme oxygenase by metalloporphyrins in primary chick embryo liver cells: evidence against a stress-mediated response
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biochemistry
dc.source.volume169
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/160
dc.identifier.contextkey578124
html.description.abstract<p>Heme oxygenase catalyzes the first and rate-controlling step in heme catabolism. One of the two forms of heme oxygenase (heme oxygenase-1) has been shown to be increased by heme, metals, and in some systems, by certain environmental stresses. However, it remains uncertain whether heme induces hepatic heme oxygenase-1 by a general stress response, or a specific heme-dependent cellular response. The work communicated here explores this issue by examining possible mechanisms whereby heme and other metalloporphyrins induce heme oxygenase-1 in normal liver cells. Primary cultures of chick embryo liver cells were tested for their ability to increase heme oxygenase mRNA after exposure to selected metalloporphyrins (heme, chromium mesoporphyrin, cobalt protoporphyrin and manganese protoporphyrin). The ability of antioxidants to decrease metalloporphyrin-mediated induction of heme oxygenase-1 mRNA was also tested. Our results indicate that: 1) the increase in heme oxygenase-1 mRNA mediated by heme or other metalloporphyrins may involve a short-lived protein(s) since the increase was prevented by several inhibitors of protein synthesis; and 2) in normal liver cells, heme-dependent oxidative stress does not play a key role in the heme-mediated induction of heme oxygenase-1. We conclude that heme and other non-heme metalloporphyrins induce heme oxygenase-1 through a mechanism requiring protein synthesis, not because metalloporphyrins increase cellular oxidative or other stress.</p>
dc.identifier.submissionpathgsbs_sp/160
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages13-20


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