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dc.contributor.authorLin, Chien-Ling
dc.contributor.authorEvans, Veronica
dc.contributor.authorShen, Shihao
dc.contributor.authorXing, Yi
dc.contributor.authorRichter, Joel D
dc.date2022-08-11T08:08:53.000
dc.date.accessioned2022-08-23T16:11:08Z
dc.date.available2022-08-23T16:11:08Z
dc.date.issued2010-02-01
dc.date.submitted2010-05-07
dc.identifier.citation<p>RNA. 2010 Feb;16(2):338-48. Epub 2009 Dec 29.</p>
dc.identifier.issn1469-9001
dc.identifier.doi10.1261/rna.1779810
dc.identifier.pmid20040591
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33094
dc.description.abstractCPEB is a sequence-specific RNA binding protein that promotes polyadenylation-induced translation in early development, during cell cycle progression and cellular senescence, and following neuronal synapse stimulation. It controls polyadenylation and translation through other interacting molecules, most notably the poly(A) polymerase Gld2, the deadenylating enzyme PARN, and the eIF4E-binding protein Maskin. Here, we report that CPEB shuttles between the nucleus and cytoplasm and that its export occurs via the CRM1-dependent pathway. In the nucleus of Xenopus oocytes, CPEB associates with lampbrush chromosomes and several proteins involved in nuclear RNA processing. CPEB also interacts with Maskin in the nucleus as well as with CPE-containing mRNAs. Although the CPE does not regulate mRNA export, it influences the degree to which mRNAs are translationally repressed in the cytoplasm. Moreover, CPEB directly or indirectly mediates the alternative splicing of at least one pre-mRNA in mouse embryo fibroblasts as well as certain mouse tissues. We propose that CPEB, together with Maskin, binds mRNA in the nucleus to ensure tight translational repression upon export to the cytoplasm. In addition, we propose that nuclear CPEB regulates specific pre-mRNA alternative splicing.
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Press
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20040591&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811663/
dc.subjectActive Transport, Cell Nucleus; Alternative Splicing; Amino Acid Sequence; Animals; Cell Line; Cell Nucleus; Female; Humans; Mice; Models, Biological; Molecular Sequence Data; NIH 3T3 Cells; Oocytes; Protein Biosynthesis; RNA Processing, Post-Transcriptional; RNA, Messenger; RNA-Binding Proteins; Recombinant Proteins; Sequence Homology, Amino Acid; Transcription Factors; Xenopus; Xenopus Proteins; mRNA Cleavage and Polyadenylation Factors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleThe nuclear experience of CPEB: implications for RNA processing and translational control.
dc.typeJournal Article
dc.source.journaltitleRNA (New York, N.Y.)
dc.source.volume16
dc.source.issue2
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2641&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1639
dc.identifier.contextkey1302429
refterms.dateFOA2022-08-23T16:11:08Z
html.description.abstract<p>CPEB is a sequence-specific RNA binding protein that promotes polyadenylation-induced translation in early development, during cell cycle progression and cellular senescence, and following neuronal synapse stimulation. It controls polyadenylation and translation through other interacting molecules, most notably the poly(A) polymerase Gld2, the deadenylating enzyme PARN, and the eIF4E-binding protein Maskin. Here, we report that CPEB shuttles between the nucleus and cytoplasm and that its export occurs via the CRM1-dependent pathway. In the nucleus of Xenopus oocytes, CPEB associates with lampbrush chromosomes and several proteins involved in nuclear RNA processing. CPEB also interacts with Maskin in the nucleus as well as with CPE-containing mRNAs. Although the CPE does not regulate mRNA export, it influences the degree to which mRNAs are translationally repressed in the cytoplasm. Moreover, CPEB directly or indirectly mediates the alternative splicing of at least one pre-mRNA in mouse embryo fibroblasts as well as certain mouse tissues. We propose that CPEB, together with Maskin, binds mRNA in the nucleus to ensure tight translational repression upon export to the cytoplasm. In addition, we propose that nuclear CPEB regulates specific pre-mRNA alternative splicing.</p>
dc.identifier.submissionpathgsbs_sp/1639
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.studentChien-Ling Lin


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