A simple method for improving the specificity of anti-methyl histone antibodies
Authors
Connor, Caroline M.Cheung, Iris
Simon, Andrew
Jakovcevski, Mira
Weng, Zhiping
Akbarian, Schahram
Student Authors
Caroline ConnorUMass Chan Affiliations
Brudnick Neuropsychiatric Research Institute, Department of PsychiatryProgram in Bioinformatics and Integrative Biology
Department of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2010-07-01Keywords
Amino Acid Sequence; Antibodies; *Antibody Specificity; Cells, Cultured; Chromatin; Chromatin Immunoprecipitation; Histones; Methylation; Transcription Initiation SiteLife Sciences
Medicine and Health Sciences
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).Source
Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's websiteDOI
10.4161/epi.5.5.11874Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33150PubMed ID
20458167Related Resources
Link to Article in PubMedRights
This is an open access article licensed under a under a Creative Commons Attribution-NonCommercial 3.0 Unported License.ae974a485f413a2113503eed53cd6c53
10.4161/epi.5.5.11874