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    A simple method for improving the specificity of anti-methyl histone antibodies

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    Authors
    Connor, Caroline M.
    Cheung, Iris
    Simon, Andrew
    Jakovcevski, Mira
    Weng, Zhiping
    Akbarian, Schahram
    Student Authors
    Caroline Connor
    UMass Chan Affiliations
    Brudnick Neuropsychiatric Research Institute, Department of Psychiatry
    Program in Bioinformatics and Integrative Biology
    Department of Biochemistry and Molecular Pharmacology
    Document Type
    Journal Article
    Publication Date
    2010-07-01
    Keywords
    Amino Acid Sequence; Antibodies; *Antibody Specificity; Cells, Cultured; Chromatin; Chromatin Immunoprecipitation; Histones; Methylation; Transcription Initiation Site
    Life Sciences
    Medicine and Health Sciences
    Neuroscience and Neurobiology
    
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    Link to Full Text
    http://dx.doi.org/10.4161/epi.5.5.11874
    Abstract
    Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).
    Source
    Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's website
    DOI
    10.4161/epi.5.5.11874
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/33150
    PubMed ID
    20458167
    Related Resources
    Link to Article in PubMed
    Rights
    This is an open access article licensed under a under a Creative Commons Attribution-NonCommercial 3.0 Unported License.
    ae974a485f413a2113503eed53cd6c53
    10.4161/epi.5.5.11874
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    Morningside Graduate School of Biomedical Sciences Scholarly Publications
    Program in Bioinformatics and Integrative Biology Publications

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