Fm1-43 labeling of Drosophila larval neuromuscular junctions
| dc.contributor.author | Ramachandran, Preethi | |
| dc.contributor.author | Budnik, Vivian | |
| dc.date | 2022-08-11T08:08:54.000 | |
| dc.date.accessioned | 2022-08-23T16:11:25Z | |
| dc.date.available | 2022-08-23T16:11:25Z | |
| dc.date.issued | 2010-08-04 | |
| dc.date.submitted | 2011-05-20 | |
| dc.identifier.citation | Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471. | |
| dc.identifier.issn | 1559-6095 (Electronic) | |
| dc.identifier.doi | 10.1101/pdb.prot5471 | |
| dc.identifier.pmid | 20679380 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/33161 | |
| dc.description.abstract | Over the last two decades, the Drosophila larval neuromuscularjunction (NMJ) has gained immense popularity as a model systemfor the study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. Here, we describe a simple methodfor looking at vesicle recycling at the NMJ by labeling withFM1-43 dye. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20679380&dopt=Abstract">Link to Article in PubMed</a> | |
| dc.relation.url | http://dx.doi.org/10.1101/pdb.prot5471 | |
| dc.subject | Animals; Dissection; Drosophila melanogaster; Larva; Neuromuscular Junction; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling | |
| dc.subject | Neuroscience and Neurobiology | |
| dc.title | Fm1-43 labeling of Drosophila larval neuromuscular junctions | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Cold Spring Harbor protocols | |
| dc.source.volume | 2010 | |
| dc.source.issue | 8 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/1700 | |
| dc.identifier.contextkey | 2022707 | |
| html.description.abstract | <p>Over the last two decades, the <em>Drosophila</em> larval neuromuscularjunction (NMJ) has gained immense popularity as a model systemfor the study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. Here, we describe a simple methodfor looking at vesicle recycling at the NMJ by labeling withFM1-43 dye.</p> | |
| dc.identifier.submissionpath | gsbs_sp/1700 | |
| dc.contributor.department | Graduate School of Biomedical Sciences, Neuroscience Program | |
| dc.contributor.department | Budnik Lab | |
| dc.contributor.department | Neurobiology | |
| dc.source.pages | pdb.prot5471 | |
| dc.contributor.student | Preethi Ramachandran |