Internalization and trafficking assay for Drosophila larvae
| dc.contributor.author | Ramachandran, Preethi | |
| dc.contributor.author | Budnik, Vivian | |
| dc.date | 2022-08-11T08:08:54.000 | |
| dc.date.accessioned | 2022-08-23T16:11:25Z | |
| dc.date.available | 2022-08-23T16:11:25Z | |
| dc.date.issued | 2010-08-04 | |
| dc.date.submitted | 2011-05-20 | |
| dc.identifier.citation | Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5472. doi: 10.1101/pdb.prot5472. | |
| dc.identifier.issn | 1559-6095 (Electronic) | |
| dc.identifier.doi | 10.1101/pdb.prot5472 | |
| dc.identifier.pmid | 20679381 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/33162 | |
| dc.description.abstract | Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This protocol describes an assaythat can be used for observing the cycling of transmembraneproteins at the plasma membrane and their trafficking withincells when there are antibodies available that bind cell-surfaceepitopes in vivo. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20679381&dopt=Abstract">Link to Article in PubMed</a> | |
| dc.relation.url | http://dx.doi.org/10.1101/pdb.prot5472 | |
| dc.subject | Animals; Biological Assay; Drosophila melanogaster; *Endocytosis; Fluorescein-5-isothiocyanate; Larva; Protein Transport | |
| dc.subject | Neuroscience and Neurobiology | |
| dc.title | Internalization and trafficking assay for Drosophila larvae | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Cold Spring Harbor protocols | |
| dc.source.volume | 2010 | |
| dc.source.issue | 8 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/1701 | |
| dc.identifier.contextkey | 2022708 | |
| html.description.abstract | <p>Over the last two decades, the <em>Drosophila</em> larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This protocol describes an assaythat can be used for observing the cycling of transmembraneproteins at the plasma membrane and their trafficking withincells when there are antibodies available that bind cell-surfaceepitopes in vivo.</p> | |
| dc.identifier.submissionpath | gsbs_sp/1701 | |
| dc.contributor.department | Graduate School of Biomedical Sciences, Neuroscience Program | |
| dc.contributor.department | Budnik Lab | |
| dc.contributor.department | Neurobiology | |
| dc.source.pages | pdb.prot5472 | |
| dc.contributor.student | Preethi Ramachandran | |
| dc.description.thesisprogram | Neuroscience |