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dc.contributor.authorRamachandran, Preethi
dc.contributor.authorBudnik, Vivian
dc.date2022-08-11T08:08:54.000
dc.date.accessioned2022-08-23T16:11:26Z
dc.date.available2022-08-23T16:11:26Z
dc.date.issued2010-08-04
dc.date.submitted2011-05-20
dc.identifier.citationCold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.
dc.identifier.issn1559-6095 (Electronic)
dc.identifier.doi10.1101/pdb.prot5474
dc.identifier.pmid20679383
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33164
dc.description.abstractOver the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20679383&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1101/pdb.prot5474
dc.subjectAnimals; Dissection; Drosophila; Larva; Microscopy, Electron; Muscles; Neuromuscular Junction
dc.subjectNeuroscience and Neurobiology
dc.titleElectron microscopy of Drosophila larval neuromuscular junctions
dc.typeJournal Article
dc.source.journaltitleCold Spring Harbor protocols
dc.source.volume2010
dc.source.issue8
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1703
dc.identifier.contextkey2022711
html.description.abstract<p>Over the last two decades, the <em>Drosophila</em> larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof <em>Drosophila</em> larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.</p>
dc.identifier.submissionpathgsbs_sp/1703
dc.contributor.departmentGraduate School of Biomedical Sciences, Neuroscience Program
dc.contributor.departmentBudnik Lab
dc.contributor.departmentNeurobiology
dc.source.pagespdb.prot5474
dc.contributor.studentPreethi Ramachandran
dc.description.thesisprogramNeuroscience


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