The MutS C terminus is essential for mismatch repair activity in vivo
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Biochemistry and Molecular Pharmacology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2005-09-15Keywords
Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; DNA Repair; DNA-Binding Proteins; Escherichia coli; *Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; *MutationLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutSdelta800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background.Source
J Bacteriol. 2005 Sep;187(18):6577-9. Link to article on publisher's siteDOI
10.1128/JB.187.18.6577-6579.2005Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33170PubMed ID
16159793Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1128/JB.187.18.6577-6579.2005