Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

Authors
Reece-Hoyes, John S.
Barutcu, A. Rasim
McCord, Rachel Patton
Jeong, Jun Seop
Jiang, Lizhi
Macwilliams, Andrew
Yang, Xinping
Salehi-Ashtiani, Kourosh
Hill, David E.
Blackshaw, Seth
Student Authors
Ahmet Rasim Barutcu
UMass Chan Affiliations
Department of Biochemistry and Molecular Pharmacology
Program in Systems Biology
Program in Molecular Medicine
Program in Gene Function and Expression
Document Type
Journal Article
Publication Date
2011-10-30
Keywords
Two-Hybrid System Techniques; High-Throughput Screening Assays; Gene Regulatory Networks; Protein Interaction Mapping; Transcription Factors
Biochemistry, Biophysics, and Structural Biology
Genetics and Genomics
Life Sciences
Medicine and Health Sciences

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Link to Full Text
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263363/pdf/nihms331259.pdf
Abstract
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
Source
Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site
DOI
10.1038/nmeth.1764
Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33227
PubMed ID
22037702
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