dSarm/Sarm1 is required for activation of an injury-induced axon death pathway
AuthorsOsterloh, Jeannette M.
Rooney, Timothy M.
Fox, A. Nicole
Powell, Eric H.
Sheehan, Amy E.
Avery, Michelle A.
Logan, Mary A.
MacDonald, Jennifer M.
Ziegenfuss, Jennifer S.
Brown, Robert H. Jr.
Freeman, Marc R.
Student AuthorsJeannette Osterloh; Timothy Rooney; Michelle Avery; Jennifer MacDonald; Jennifer Zeigenfuss
UMass Chan AffiliationsGraduate School of Biomedical Sciences, MD/PhD Program
Graduate School of Biomedical Sciences, Neuroscience Program
Department of Neurology
Department of Neurobiology
KeywordsAnimals; Animals, Genetically Modified; Apoptosis; Armadillo Domain Proteins; Axons; Axotomy; Cell Survival; Cells, Cultured; Cytoskeletal Proteins; Denervation; Drosophila; Drosophila Proteins; Mice; Mutation; Neurons; Sciatic Nerve; Signal Transduction; Superior Cervical Ganglion; Tissue Culture Techniques; *Wallerian Degeneration
Neuroscience and Neurobiology
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AbstractAxonal and synaptic degeneration is a hallmark of peripheral neuropathy, brain injury, and neurodegenerative disease. Axonal degeneration has been proposed to be mediated by an active autodestruction program, akin to apoptotic cell death; however, loss-of-function mutations capable of potently blocking axon self-destruction have not been described. Here, we show that loss of the Drosophila Toll receptor adaptor dSarm (sterile alpha/Armadillo/Toll-Interleukin receptor homology domain protein) cell-autonomously suppresses Wallerian degeneration for weeks after axotomy. Severed mouse Sarm1 null axons exhibit remarkable long-term survival both in vivo and in vitro, indicating that Sarm1 prodegenerative signaling is conserved in mammals. Our results provide direct evidence that axons actively promote their own destruction after injury and identify dSarm/Sarm1 as a member of an ancient axon death signaling pathway.
Science. 2012 Jul 27;337(6093):481-4. Epub 2012 Jun 7. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/33255
Related ResourcesLink to Article in PubMed