• Login
    View Item 
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside Graduate School of Biomedical Sciences Scholarly Publications
    • View Item
    •   Home
    • UMass Chan Student Research and Publications
    • Morningside Graduate School of Biomedical Sciences
    • Morningside Graduate School of Biomedical Sciences Scholarly Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Campellone, Kenneth Geno
    Roe, Andrew J.
    Lobner-Olesen, Anders
    Murphy, Kenan C.
    Magoun, Loranne
    Brady, Michael John
    Donohue-Rolfe, Arthur
    Tzipori, Saul
    Gally, David L.
    Leong, John M.
    Marinus, Martin G.
    Show allShow less
    UMass Chan Affiliations
    Department of Molecular Genetics and Microbiology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2007-02-17
    Keywords
    Actins; Adhesins, Bacterial; Animals; Artificial Gene Fusion; *Bacterial Adhesion; Carrier Proteins; Disease Models, Animal; Escherichia coli Infections; Escherichia coli O157; Escherichia coli Proteins; *Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Reporter; Green Fluorescent Proteins; Hela Cells; Humans; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Oligonucleotide Array Sequence Analysis; RNA, Bacterial; RNA, Messenger; Receptors, Cell Surface; Reverse Transcriptase Polymerase Chain Reaction; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Swine; Transcription, Genetic
    Life Sciences
    Medicine and Health Sciences
    
    Metadata
    Show full item record
    Link to Full Text
    http://dx.doi.org/10.1111/j.1365-2958.2007.05602.x
    Abstract
    Enterohaemorrhagic Escherichia coli (EHEC) are highly infectious pathogens capable of causing severe diarrhoeal illnesses. As a critical step during their colonization, EHEC adhere intimately to intestinal epithelial cells and generate F-actin 'pedestal' structures that elevate them above surrounding cell surfaces. Intimate adhesion and pedestal formation result from delivery of the EHEC type III secretion system (TTSS) effector proteins Tir and EspF(U) into the host cell and expression of the bacterial outer membrane adhesin, intimin. To investigate a role for DNA methylation during the regulation of adhesion and pedestal formation in EHEC, we deleted the dam (DNA adenine methyltransferase) gene from EHEC O157:H7 and demonstrate that this mutation results in increased interactions with cultured host cells. EHECDeltadam exhibits dramatically elevated levels of adherence and pedestal formation when compared with wild-type EHEC, and expresses significantly higher protein levels of intimin, Tir and EspF(U). Analyses of GFP fusions, Northern blotting, reverse transcription polymerase chain reaction, and microarray experiments indicate that the abundance of Tir in the dam mutant is not due to increased transcription levels, raising the possibility that Dam methylation can indirectly control protein expression by a post-transcriptional mechanism. In contrast to other dam-deficient pathogens, EHECDeltadam is capable of robust intestinal colonization of experimentally infected animals.
    Source
    Mol Microbiol. 2007 Mar;63(5):1468-81. Link to article on publisher's site
    DOI
    10.1111/j.1365-2958.2007.05602.x
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/33256
    PubMed ID
    17302821
    Related Resources
    Link to article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1365-2958.2007.05602.x
    Scopus Count
    Collections
    Morningside Graduate School of Biomedical Sciences Scholarly Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.