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dc.contributor.authorMallick, Emily M.
dc.contributor.authorMcBee, Megan E.
dc.contributor.authorVanguri, Vijay K.
dc.contributor.authorMelton-Celsa, Angela R.
dc.contributor.authorSchlieper, Katherine
dc.contributor.authorKaralius, Brad J.
dc.contributor.authorO’Brien, Alison D.
dc.contributor.authorButterton, Joan R.
dc.contributor.authorLeong, John M.
dc.contributor.authorSchauer, David B.
dc.date2022-08-11T08:08:54.000
dc.date.accessioned2022-08-23T16:11:51Z
dc.date.available2022-08-23T16:11:51Z
dc.date.issued2012-11-01
dc.date.submitted2012-11-13
dc.identifier.citationMallick EM, McBee ME, Vanguri VK, Melton-Celsa AR, Schlieper K, Karalius BJ, O'Brien AD, Butterton JR, Leong JM, Schauer DB. A novel murine infection model for Shiga toxin-producing Escherichia coli. J Clin Invest. 2012;122(11):4012–4024. doi:10.1172/JCI62746. <a href="http://dx.doi.org/10.1172/JCI62746">Link to article on publisher's website</a>
dc.identifier.doi10.1172/JCI62746
dc.identifier.pmid23041631
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33265
dc.description.abstractEnterohemorrhagic E. coli (EHEC) is an important subset of Shiga toxin-producing (Stx-producing) E. coli (STEC), pathogens that have been implicated in outbreaks of food-borne illness and can cause intestinal and systemic disease, including severe renal damage. Upon attachment to intestinal epithelium, EHEC generates "attaching and effacing" (AE) lesions characterized by intimate attachment and actin rearrangement upon host cell binding. Stx produced in the gut transverses the intestinal epithelium, causing vascular damage that leads to systemic disease. Models of EHEC infection in conventional mice do not manifest key features of disease, such as AE lesions, intestinal damage, and systemic illness. In order to develop an infection model that better reflects the pathogenesis of this subset of STEC, we constructed an Stx-producing strain of Citrobacter rodentium, a murine AE pathogen that otherwise lacks Stx. Mice infected with Stx-producing C. rodentium developed AE lesions on the intestinal epithelium and Stx-dependent intestinal inflammatory damage. Further, the mice experienced lethal infection characterized by histopathological and functional kidney damage. The development of a murine model that encompasses AE lesion formation and Stx-mediated tissue damage will provide a new platform upon which to identify EHEC alterations of host epithelium that contribute to systemic disease.
dc.language.isoen_US
dc.publisherAmerican Society for Clinical Investigation
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=23041631&dopt=Abstract">Link to article in PubMed</a>
dc.rights<p>Copyright © 2012, American Society for Clinical Investigation. Publisher PDF posted as allowed by the publisher's author rights policy at http://static.the-jci.org/content_assets/admin/forms/jcicopyright.pdf.</p>
dc.subjectEnterohemorrhagic Escherichia coli; Shiga-Toxigenic Escherichia coli; Citrobacter rodentium; Mice; Models, Animal
dc.subjectshiga toxin
dc.subjectcitrobacter rodentium
dc.subjectehec
dc.subjecthus
dc.subjectBacterial Infections and Mycoses
dc.subjectDigestive System Diseases
dc.subjectMicrobiology
dc.titleA novel murine infection model for Shiga toxin-producing Escherichia coli
dc.typeJournal Article
dc.source.journaltitleJournal of Clinical Investigation
dc.source.volume122
dc.source.issue11
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2817&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1798
dc.identifier.contextkey3465547
refterms.dateFOA2022-08-23T16:11:51Z
html.description.abstract<p>Enterohemorrhagic E. coli (EHEC) is an important subset of Shiga toxin-producing (Stx-producing) E. coli (STEC), pathogens that have been implicated in outbreaks of food-borne illness and can cause intestinal and systemic disease, including severe renal damage. Upon attachment to intestinal epithelium, EHEC generates "attaching and effacing" (AE) lesions characterized by intimate attachment and actin rearrangement upon host cell binding. Stx produced in the gut transverses the intestinal epithelium, causing vascular damage that leads to systemic disease. Models of EHEC infection in conventional mice do not manifest key features of disease, such as AE lesions, intestinal damage, and systemic illness. In order to develop an infection model that better reflects the pathogenesis of this subset of STEC, we constructed an Stx-producing strain of Citrobacter rodentium, a murine AE pathogen that otherwise lacks Stx. Mice infected with Stx-producing C. rodentium developed AE lesions on the intestinal epithelium and Stx-dependent intestinal inflammatory damage. Further, the mice experienced lethal infection characterized by histopathological and functional kidney damage. The development of a murine model that encompasses AE lesion formation and Stx-mediated tissue damage will provide a new platform upon which to identify EHEC alterations of host epithelium that contribute to systemic disease.</p>
dc.identifier.submissionpathgsbs_sp/1798
dc.contributor.departmentDepartment of Pathology
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.source.pages4012-4024
dc.contributor.studentEmily M. Mallick


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