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dc.contributor.authorConine, Colin C.
dc.contributor.authorMoresco, James J.
dc.contributor.authorGu, Weifeng
dc.contributor.authorShirayama, Masaki
dc.contributor.authorConte, Darryl Jr.
dc.contributor.authorYates, John R. III
dc.contributor.authorMello, Craig C.
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:03Z
dc.date.available2022-08-23T16:12:03Z
dc.date.issued2013-12-19
dc.date.submitted2014-01-07
dc.identifier.citationConine, C.C., Moresco, J.J., Gu, W., Shirayama, M., Conte, D., Jr., Yates, J.R., 3rd, and Mello, C.C. (2013). Argonautes Promote Male Fertility and Provide a Paternal Memory of Germline Gene Expression in C. elegans. Cell 155(7):1532-1544. doi: 10.1016/j.cell.2013.11.032. <a href="http://dx.doi.org/10.1016/j.cell.2013.11.032">Link to article on publisher's website</a>
dc.identifier.doi10.1016/j.cell.2013.11.032
dc.identifier.pmid24360276
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33315
dc.description.abstractDuring each life cycle, germ cells preserve and pass on both genetic and epigenetic information. In C. elegans, the ALG-3/4 Argonaute proteins are expressed during male gametogenesis and promote male fertility. Here, we show that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis. Our findings suggest that ALG-3/4 functions during spermatogenesis to amplify a small RNA signal that represents an epigenetic memory of male-specific gene expression. CSR-1, which is abundant in mature sperm, appears to transmit this memory to offspring. Surprisingly, in addition to small RNAs targeting male-specific genes, we show that males also harbor an extensive repertoire of CSR-1 small RNAs targeting oogenesis-specific mRNAs. Together, these findings suggest that C. elegans sperm transmit not only the genome but also epigenetic binary signals in the form of Argonaute/small RNA complexes that constitute a memory of gene expression in preceding generations.
dc.language.isoen_US
dc.publisherCell Press
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24360276&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.cell.2013.11.032
dc.subjectEpigenetics
dc.subjectRNAi
dc.subjectSmall RNA
dc.subjectArgonaute
dc.subjectGermline Sperm
dc.subjectBioinformatics
dc.subjectBiology
dc.subjectCell Biology
dc.subjectDevelopmental Biology
dc.subjectGenetics
dc.subjectGenomics
dc.subjectMolecular Genetics
dc.titleArgonautes Promote Male Fertility and Provide a Paternal Memory of Germline Gene Expression in C. elegans
dc.typeJournal Article
dc.source.journaltitleCell
dc.source.volume155
dc.source.issue7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2862&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1843
dc.identifier.contextkey4966509
refterms.dateFOA2022-08-23T16:12:03Z
html.description.abstract<p>During each life cycle, germ cells preserve and pass on both genetic and epigenetic information. In C. elegans, the ALG-3/4 Argonaute proteins are expressed during male gametogenesis and promote male fertility. Here, we show that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis. Our findings suggest that ALG-3/4 functions during spermatogenesis to amplify a small RNA signal that represents an epigenetic memory of male-specific gene expression. CSR-1, which is abundant in mature sperm, appears to transmit this memory to offspring. Surprisingly, in addition to small RNAs targeting male-specific genes, we show that males also harbor an extensive repertoire of CSR-1 small RNAs targeting oogenesis-specific mRNAs. Together, these findings suggest that C. elegans sperm transmit not only the genome but also epigenetic binary signals in the form of Argonaute/small RNA complexes that constitute a memory of gene expression in preceding generations.</p>
dc.identifier.submissionpathgsbs_sp/1843
dc.contributor.departmentRNA Therapeutics Institute and Program in Molecular Medicine
dc.source.pages1532-1544
dc.contributor.studentColin C. Conine


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