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dc.contributor.authorHuang, Tingting
dc.contributor.authorRivera-Perez, Jaime A.
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:06Z
dc.date.available2022-08-23T16:12:06Z
dc.date.issued2014-03-28
dc.date.submitted2014-04-11
dc.identifier.citationHuang T, Rivera-Pérez JA. Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence. Genesis. 2014 Feb 25. doi: 10.1002/dvg.22761. <a href="http://dx.doi.org/10.1002/dvg.22761">Link to article on publisher's website</a>
dc.identifier.issn1526-968X
dc.identifier.doi10.1002/dvg.22761
dc.identifier.pmid24616249
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33326
dc.description.abstractSenescence-associated β-galactosidase (SA-β-gal) activity is widely used as a marker of cellular senescence and as an indicator of organismal aging. Here, we report that SA-β-gal activity is present in the visceral endoderm layer of early postimplantation mouse embryos in predictable patterns that vary as the embryo progresses in development. However, determination of the mitotic index and analysis of the expression of Cdkn1a (p21), a marker of senescent cells, do not indicate cellular senescence. Instead, analysis of embryos in culture revealed the presence of SA-β-gal activity in apical vacuoles of visceral endoderm cells likely a reflection of acidic β-galactosidase function in these organelles. SA-β-gal serves as a practical marker of the dynamics of the visceral endoderm that can be applied to developmental as well as functional studies of early mammalian embryos.
dc.language.isoen_US
dc.publisherWiley-Liss
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24616249&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/dvg.22761
dc.subjectSA-β-gal
dc.subjectapical vacuoles
dc.subjectembryo
dc.subjectmouse
dc.subjectyolk sac
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.titleSenescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence
dc.typeJournal Article
dc.source.journaltitleGenesis
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1853
dc.identifier.contextkey5472287
html.description.abstract<p>Senescence-associated β-galactosidase (SA-β-gal) activity is widely used as a marker of cellular senescence and as an indicator of organismal aging. Here, we report that SA-β-gal activity is present in the visceral endoderm layer of early postimplantation mouse embryos in predictable patterns that vary as the embryo progresses in development. However, determination of the mitotic index and analysis of the expression of Cdkn1a (p21), a marker of senescent cells, do not indicate cellular senescence. Instead, analysis of embryos in culture revealed the presence of SA-β-gal activity in apical vacuoles of visceral endoderm cells likely a reflection of acidic β-galactosidase function in these organelles. SA-β-gal serves as a practical marker of the dynamics of the visceral endoderm that can be applied to developmental as well as functional studies of early mammalian embryos.</p>
dc.identifier.submissionpathgsbs_sp/1853
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.contributor.studentTingting Huang


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