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dc.contributor.authorMou, Haiwei
dc.contributor.authorKennedy, Zachary
dc.contributor.authorAnderson, Daniel G.
dc.contributor.authorYin, Hao
dc.contributor.authorXue, Wen
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:12Z
dc.date.available2022-08-23T16:12:12Z
dc.date.issued2015-01-01
dc.date.submitted2015-06-18
dc.identifier.citation<p>Mou H, Kennedy Z, Anderson DG, Yin H, Xue W. Precision cancer mouse models through genome editing with CRISPR-Cas9. Genome Med. 2015 Jun 9;7(1):53. doi: 10.1186/s13073-015-0178-7. eCollection 2015. Review. PubMed PMID: 26060510; PubMed Central PMCID: PMC4460969. <a href="http://dx.doi.org/10.1186/s13073-015-0178-7" target="_blank">Link to article on publisher's site</a></p>
dc.identifier.issn1756-994X
dc.identifier.doi10.1186/s13073-015-0178-7
dc.identifier.pmid26060510
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33346
dc.description.abstractThe cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse-model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=26060510&dopt=Abstract">Link to article in PubMed</a>
dc.rights© 2015 Mou et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcancer
dc.subjectgenome editing
dc.subjectgenome engineering
dc.subjectmouse models
dc.subjectCRISPR-Cas9
dc.subjectBioinformatics
dc.subjectCancer Biology
dc.subjectComputational Biology
dc.subjectGenomics
dc.subjectMolecular Genetics
dc.titlePrecision cancer mouse models through genome editing with CRISPR-Cas9
dc.typeJournal Article
dc.source.journaltitleGenome Medicine
dc.source.volume7
dc.source.issue1
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2893&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1872
dc.legacy.embargo2015-06-09T00:00:00-07:00
dc.identifier.contextkey7232357
refterms.dateFOA2022-08-23T16:12:12Z
html.description.abstract<p>The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse-model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine.</p>
dc.identifier.submissionpathgsbs_sp/1872
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentRNA Therapeutics Institute
dc.source.pages53
dc.contributor.studentZachary Kennedy


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© 2015 Mou et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as © 2015 Mou et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.