Noncoding RNA. piRNA-guided transposon cleavage initiates Zucchini-dependent, phased piRNA production
Student Authors
Bo W. Han; Wei WangUMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDepartment of Biochemistry and Molecular Pharmacology
RNA Therapeutics Institute
Document Type
Journal ArticlePublication Date
2015-05-15Keywords
Animals; Argonaute Proteins; Drosophila Proteins; Drosophila melanogaster; Endoribonucleases; Female; Germ Cells; Male; Metabolic Networks and Pathways; Mice; Ovary; Peptide Initiation Factors; *RNA Cleavage; RNA, Guide; RNA, Small Interfering; *Retroelements; TestisBiochemistry
Bioinformatics
Genetics and Genomics
Metadata
Show full item recordAbstract
PIWI-interacting RNAs (piRNAs) protect the animal germ line by silencing transposons. Primary piRNAs, generated from transcripts of genomic transposon "junkyards" (piRNA clusters), are amplified by the "ping-pong" pathway, yielding secondary piRNAs. We report that secondary piRNAs, bound to the PIWI protein Ago3, can initiate primary piRNA production from cleaved transposon RNAs. The first ~26 nucleotides (nt) of each cleaved RNA becomes a secondary piRNA, but the subsequent ~26 nt become the first in a series of phased primary piRNAs that bind Piwi, allowing piRNAs to spread beyond the site of RNA cleavage. The ping-pong pathway increases only the abundance of piRNAs, whereas production of phased primary piRNAs from cleaved transposon RNAs adds sequence diversity to the piRNA pool, allowing adaptation to changes in transposon sequence.Source
Science. 2015 May 15;348(6236):817-21. doi: 10.1126/science.aaa1264. Link to article on publisher's siteDOI
10.1126/science.aaa1264Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33358PubMed ID
25977554Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1126/science.aaa1264