High-resolution chromatin dynamics during a yeast stress response
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Authors
Weiner, AssafHsieh, Tsung-Han S.
Appleboim, Alon
Chen, Hsiuyi V.
Rahat, Ayelet
Amit, Ido
Rando, Oliver J.
Friedman, Nir
Student Authors
Tsung-Han S. Hsieh; Hsiuyi V. ChenUMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2015-04-16Keywords
Chromatin; Chromatin Assembly and Disassembly; Diamide; Gene Expression Regulation, Fungal; Genome, Fungal; Histones; Molecular Sequence Data; Saccharomyces cerevisiae; Transcription, GeneticGenomics
Molecular Genetics
Metadata
Show full item recordAbstract
Covalent histone modifications are highly conserved and play multiple roles in eukaryotic transcription regulation. Here, we mapped 26 histone modifications genome-wide in exponentially growing yeast and during a dramatic transcriptional reprogramming-the response to diamide stress. We extend prior studies showing that steady-state histone modification patterns reflect genomic processes, especially transcription, and display limited combinatorial complexity. Interestingly, during the stress response we document a modest increase in the combinatorial complexity of histone modification space, resulting from roughly 3% of all nucleosomes transiently populating rare histone modification states. Most of these rare histone states result from differences in the kinetics of histone modification that transiently uncouple highly correlated marks, with slow histone methylation changes often lagging behind the more rapid acetylation changes. Explicit analysis of modification dynamics uncovers ordered sequences of events in gene activation and repression. Together, our results provide a comprehensive view of chromatin dynamics during a massive transcriptional upheaval.Source
Mol Cell. 2015 Apr 16;58(2):371-86. doi: 10.1016/j.molcel.2015.02.002. Epub 2015 Mar 19. Link to article on publisher's siteDOI
10.1016/j.molcel.2015.02.002Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33362PubMed ID
25801168Related Resources
Link to Article in PubMedRights
Open Access funded by European Research Council. Under a Creative Commons license
Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.molcel.2015.02.002
Scopus Count
Collections
Except where otherwise noted, this item's license is described as <p>Open Access funded by European Research Council. Under a Creative Commons <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/">license</a></p>