High-resolution chromatin dynamics during a yeast stress response
Hsieh, Tsung-Han S.
Chen, Hsiuyi V.
Rando, Oliver J.
Student AuthorsTsung-Han S. Hsieh; Hsiuyi V. Chen
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Document TypeJournal Article
KeywordsChromatin; Chromatin Assembly and Disassembly; Diamide; Gene Expression Regulation, Fungal; Genome, Fungal; Histones; Molecular Sequence Data; Saccharomyces cerevisiae; Transcription, Genetic
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AbstractCovalent histone modifications are highly conserved and play multiple roles in eukaryotic transcription regulation. Here, we mapped 26 histone modifications genome-wide in exponentially growing yeast and during a dramatic transcriptional reprogramming-the response to diamide stress. We extend prior studies showing that steady-state histone modification patterns reflect genomic processes, especially transcription, and display limited combinatorial complexity. Interestingly, during the stress response we document a modest increase in the combinatorial complexity of histone modification space, resulting from roughly 3% of all nucleosomes transiently populating rare histone modification states. Most of these rare histone states result from differences in the kinetics of histone modification that transiently uncouple highly correlated marks, with slow histone methylation changes often lagging behind the more rapid acetylation changes. Explicit analysis of modification dynamics uncovers ordered sequences of events in gene activation and repression. Together, our results provide a comprehensive view of chromatin dynamics during a massive transcriptional upheaval.
SourceMol Cell. 2015 Apr 16;58(2):371-86. doi: 10.1016/j.molcel.2015.02.002. Epub 2015 Mar 19. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/33362
Related ResourcesLink to Article in PubMed
Open Access funded by European Research Council. Under a Creative Commons license
Except where otherwise noted, this item's license is described as <p>Open Access funded by European Research Council. Under a Creative Commons <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/">license</a></p>