Clot injection technique affects thrombolytic efficacy in a rat embolic stroke model: implications for translaboratory collaborations
Student Authors
Juyu Chueh; Nils HenningerDocument Type
Journal ArticlePublication Date
2014-04-01Keywords
Animals; Cerebrovascular Circulation; *Clinical Laboratory Techniques; Cooperative Behavior; *Disease Models, Animal; Fibrinolytic Agents; Infarction, Middle Cerebral Artery; Injections, Intra-Arterial; Intracranial Embolism; Male; Rats; Rats, Inbred WKY; Stroke; Tissue Plasminogen Activator; Treatment OutcomeNervous System Diseases
Neurology
Radiology
Metadata
Show full item recordAbstract
Current recommendations encourage the use of embolic stroke (ES) models and replication of results across laboratories in preclinical research. Since such endeavors employ different surgeons, we sought to ascertain the impact of injection technique on outcome and response to thrombolysis in an ES model. Embolic stroke was induced in Male Wistar Kyoto rats (n=166) by a fast or a slow clot injection (CI) technique. Saline or recombinant tissue plasminogen activator (rtPA) was given at 1 hour after stroke. Flow rate curves were assessed in 24 animals. Cerebral perfusion was assessed using laser Doppler flowmetry. Edema corrected infarct volume, hemispheric swelling, hemorrhagic transformation, and neurologic outcome were assessed at 24 hours after stroke. Clot burden was estimated in a subset of animals (n=40). Slow CI resulted in significantly smaller infarct volumes (P=0.024) and better neurologic outcomes (P=0.01) compared with fast CI at 24 hours. Unexpectedly, rtPA treatment attenuated infarct size in fast (P<0.001) but not in slow CI experiments (P=0.382), possibly related to reperfusion injury as indicated by greater hemorrhagic transformation (P < 0.001) and hemispheric swelling (P < 0.05). Outcome and response to thrombolysis after ES are operator dependent, which needs to be considered when comparing results obtained from different laboratories.Source
J Cereb Blood Flow Metab. 2014 Apr;34(4):677-82. doi: 10.1038/jcbfm.2014.1. Epub 2014 Jan 15. Link to article on publisher's siteDOI
10.1038/jcbfm.2014.1Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33382PubMed ID
24424380Notes
Co-author Nils Henninger is a doctoral student in the Millennium PhD Program (MPP) in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/jcbfm.2014.1