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dc.contributor.authorKok, Fatma O.
dc.contributor.authorGupta, Ankit
dc.contributor.authorLawson, Nathan D.
dc.contributor.authorWolfe, Scot A.
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:24Z
dc.date.available2022-08-23T16:12:24Z
dc.date.issued2014-01-01
dc.date.submitted2015-08-31
dc.identifier.citationMethods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. <a href="http://dx.doi.org/10.1007/978-1-62703-721-1_13">Link to article on publisher's site</a>
dc.identifier.issn1064-3745 (Linking)
dc.identifier.doi10.1007/978-1-62703-721-1_13
dc.identifier.pmid24233786
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33387
dc.description.abstractArtificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24233786&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1007/978-1-62703-721-1_13
dc.subjectAnimals; Animals, Genetically Modified; Base Sequence; DNA Primers; Deoxyribonucleases; Escherichia coli; Gene Targeting; *INDEL Mutation; Molecular Sequence Data; Protein Engineering; Substrate Specificity; Zebrafish; Zinc Fingers
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectMolecular Biology
dc.titleConstruction and application of site-specific artificial nucleases for targeted gene editing
dc.typeBook Chapter
dc.source.booktitleMethods in molecular biology (Clifton, N.J.)
dc.source.volume1101
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1912
dc.identifier.contextkey7536287
html.description.abstract<p>Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.</p>
dc.identifier.submissionpathgsbs_sp/1912
dc.contributor.departmentProgram in Gene Function and Expression
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pages267-303
dc.contributor.studentAnkit Gupta


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