Distinct Notch signaling outputs pattern the developing arterial system
Authors
Quillien, AurelieMoore, John C.
Shin, Masahiro
Siekmann, Arndt F.
Smith, Tom
Pan, Luyuan
Moens, Cecilia B.
Parsons, Michael J.
Lawson, Nathan D.
Student Authors
John C. MooreUMass Chan Affiliations
Program in Gene Function and ExpressionDocument Type
Journal ArticlePublication Date
2014-04-01Keywords
Animals; Animals, Genetically Modified; Arteries; Body Patterning; Cell Differentiation; Embryo, Nonmammalian; Endothelium, Vascular; Morphogenesis; Neovascularization, Physiologic; Receptors, Notch; Signal Transduction; Veins; ZebrafishCell and Developmental Biology
Developmental Biology
Metadata
Show full item recordAbstract
Differentiation of arteries and veins is essential for the development of a functional circulatory system. In vertebrate embryos, genetic manipulation of Notch signaling has demonstrated the importance of this pathway in driving artery endothelial cell differentiation. However, when and where Notch activation occurs to affect endothelial cell fate is less clear. Using transgenic zebrafish bearing a Notch-responsive reporter, we demonstrate that Notch is activated in endothelial progenitors during vasculogenesis prior to blood vessel morphogenesis and is maintained in arterial endothelial cells throughout larval stages. Furthermore, we find that endothelial progenitors in which Notch is activated are committed to a dorsal aorta fate. Interestingly, some arterial endothelial cells subsequently downregulate Notch signaling and then contribute to veins during vascular remodeling. Lineage analysis, together with perturbation of both Notch receptor and ligand function, further suggests several distinct developmental windows in which Notch signaling acts to promote artery commitment and maintenance. Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis.Source
Development. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 2014 Mar 5. Link to article on publisher's siteDOI
10.1242/dev.099986Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33389PubMed ID
24598161Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1242/dev.099986