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dc.contributor.authorQuillien, Aurelie
dc.contributor.authorMoore, John C.
dc.contributor.authorShin, Masahiro
dc.contributor.authorSiekmann, Arndt F.
dc.contributor.authorSmith, Tom
dc.contributor.authorPan, Luyuan
dc.contributor.authorMoens, Cecilia B.
dc.contributor.authorParsons, Michael J.
dc.contributor.authorLawson, Nathan D.
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:25Z
dc.date.available2022-08-23T16:12:25Z
dc.date.issued2014-04-01
dc.date.submitted2015-08-31
dc.identifier.citationDevelopment. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 2014 Mar 5. <a href="http://dx.doi.org/10.1242/dev.099986">Link to article on publisher's site</a>
dc.identifier.issn0950-1991 (Linking)
dc.identifier.doi10.1242/dev.099986
dc.identifier.pmid24598161
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33389
dc.description.abstractDifferentiation of arteries and veins is essential for the development of a functional circulatory system. In vertebrate embryos, genetic manipulation of Notch signaling has demonstrated the importance of this pathway in driving artery endothelial cell differentiation. However, when and where Notch activation occurs to affect endothelial cell fate is less clear. Using transgenic zebrafish bearing a Notch-responsive reporter, we demonstrate that Notch is activated in endothelial progenitors during vasculogenesis prior to blood vessel morphogenesis and is maintained in arterial endothelial cells throughout larval stages. Furthermore, we find that endothelial progenitors in which Notch is activated are committed to a dorsal aorta fate. Interestingly, some arterial endothelial cells subsequently downregulate Notch signaling and then contribute to veins during vascular remodeling. Lineage analysis, together with perturbation of both Notch receptor and ligand function, further suggests several distinct developmental windows in which Notch signaling acts to promote artery commitment and maintenance. Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24598161&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074308/
dc.subjectAnimals; Animals, Genetically Modified; Arteries; Body Patterning; Cell Differentiation; Embryo, Nonmammalian; Endothelium, Vascular; Morphogenesis; Neovascularization, Physiologic; Receptors, Notch; Signal Transduction; Veins; Zebrafish
dc.subjectCell and Developmental Biology
dc.subjectDevelopmental Biology
dc.titleDistinct Notch signaling outputs pattern the developing arterial system
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume141
dc.source.issue7
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1915
dc.identifier.contextkey7536291
html.description.abstract<p>Differentiation of arteries and veins is essential for the development of a functional circulatory system. In vertebrate embryos, genetic manipulation of Notch signaling has demonstrated the importance of this pathway in driving artery endothelial cell differentiation. However, when and where Notch activation occurs to affect endothelial cell fate is less clear. Using transgenic zebrafish bearing a Notch-responsive reporter, we demonstrate that Notch is activated in endothelial progenitors during vasculogenesis prior to blood vessel morphogenesis and is maintained in arterial endothelial cells throughout larval stages. Furthermore, we find that endothelial progenitors in which Notch is activated are committed to a dorsal aorta fate. Interestingly, some arterial endothelial cells subsequently downregulate Notch signaling and then contribute to veins during vascular remodeling. Lineage analysis, together with perturbation of both Notch receptor and ligand function, further suggests several distinct developmental windows in which Notch signaling acts to promote artery commitment and maintenance. Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis.</p>
dc.identifier.submissionpathgsbs_sp/1915
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages1544-52
dc.contributor.studentJohn C. Moore


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