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dc.contributor.authorKerr, Kimberly S.
dc.contributor.authorFuentes Medel, Yuly F.
dc.contributor.authorBrewer, Cassandra
dc.contributor.authorBarria, Romina
dc.contributor.authorAshley, James A.
dc.contributor.authorAbruzzi, Katharine C.
dc.contributor.authorSheehan, Amy E.
dc.contributor.authorTasdemir, Ozge E.
dc.contributor.authorFreeman, Marc R.
dc.contributor.authorBudnik, Vivian
dc.date2022-08-11T08:08:55.000
dc.date.accessioned2022-08-23T16:12:26Z
dc.date.available2022-08-23T16:12:26Z
dc.date.issued2014-02-19
dc.date.submitted2015-09-21
dc.identifier.citationJ Neurosci. 2014 Feb 19;34(8):2910-20. doi: 10.1523/JNEUROSCI.3714-13.2014. <a href="http://dx.doi.org/10.1523/JNEUROSCI.3714-13.2014">Link to article on publisher's site</a>
dc.identifier.issn0270-6474 (Linking)
dc.identifier.doi10.1523/JNEUROSCI.3714-13.2014
dc.identifier.pmid24553932
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33396
dc.description.abstractGlial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24553932&dopt=Abstract">Link to Article in PubMed</a>
dc.rightsPublisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright. Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International (CC BY 4.0) license</a>.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals; Chromatin Immunoprecipitation; Drosophila; Drosophila Proteins; Electrophysiological Processes; Homeodomain Proteins; Image Processing, Computer-Assisted; Immunohistochemistry; Microscopy, Confocal; Neuroglia; Neuromuscular Junction; RNA Interference; Real-Time Polymerase Chain Reaction; Receptors, Glutamate; Synapses; Transfection; Wnt Proteins
dc.subjectDrosophila
dc.subjectNMJ
dc.subjectglia
dc.subjectsynapse
dc.subjectwnt/Wg
dc.subjectDevelopmental Neuroscience
dc.titleGlial wingless/Wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction
dc.typeJournal Article
dc.source.journaltitleThe Journal of neuroscience : the official journal of the Society for Neuroscience
dc.source.volume34
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2942&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/1921
dc.identifier.contextkey7622773
refterms.dateFOA2022-08-23T16:12:27Z
html.description.abstract<p>Glial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.</p>
dc.identifier.submissionpathgsbs_sp/1921
dc.contributor.departmentGraduate School of Biomedical Sciences, Neuroscience Program
dc.contributor.departmentBudnik Lab
dc.contributor.departmentFreeman Lab
dc.contributor.departmentNeurobiology
dc.source.pages2910-20
dc.contributor.studentKimberly S. Kerr
dc.contributor.studentYuly F. Fuentes-Medel
dc.contributor.studentOzge E. Tasdemir
dc.contributor.studentJames Ashley
dc.description.thesisprogramNeuroscience


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Publisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright. Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International (CC BY 4.0) license</a>.
Except where otherwise noted, this item's license is described as Publisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright. Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a <a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International (CC BY 4.0) license</a>.