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dc.contributor.authorChen, Yin Ching I.
dc.contributor.authorGalpern, Wendy R.
dc.contributor.authorBrownell, Anna-Liisa
dc.contributor.authorMatthews, Russell T.
dc.contributor.authorBogdanov, Mikhail
dc.contributor.authorIsacson, Ole
dc.contributor.authorKeltner, John R.
dc.contributor.authorBeal, M. Flint
dc.contributor.authorRosen, Bruce R.
dc.contributor.authorJenkins, Bruce G.
dc.date2022-08-11T08:08:56.000
dc.date.accessioned2022-08-23T16:12:57Z
dc.date.available2022-08-23T16:12:57Z
dc.date.issued1997-10-27
dc.date.submitted2008-08-19
dc.identifier.citation<p>Magn Reson Med. 1997 Sep;38(3):389-98.</p>
dc.identifier.issn0740-3194 (Print)
dc.identifier.doi10.1002/mrm.1910380306
dc.identifier.pmid9339439
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33505
dc.description.abstractThe metabolic activation resulting from direct dopaminergic stimulation can be detected using auto-radiography, positron emission tomography (PET) or, potentially, fMRI techniques. To establish the validity of the latter possibility, we have performed a number of experiments. We measured the regional selectivity of two different dopaminergic ligands: the dopamine release compound D-amphetamine and the dopamine transporter antagonist 2 beta-carbomethoxy-3 beta-(4-fluoropheny) tropane (CFT). Both compounds led to increased signal intensity in gradient echo images in regions of the brain with high dopamine receptor density (frontal cortex, striatum, cingulate cortex > > parietal cortex). Lesioning the animals with unilaterally administered 6-hydroxydopamine (6-OHDA) led to ablation of the phMRI response on the ipsilateral side; control measurements of rCBV and rCBF using bolus injections of Gd-DTPA showed that the baseline rCBV and rCBF values were intact on the lesioned side. The time course of the BOLD signal changes paralleled the changes observed by microdialysis measurements of dopamine release in the striatum for both amphetamine and CFT; peaking at 20-40 min after injection and returning to baseline at about 70-90 min. Signal changes were not correlated with either heart rate, blood pressure or pCO2. Measurement of PET binding in the same animals showed an excellent correlation with the phMRI data when compared by either measurements of the number of pixels activated or percent signal change in a given region. The time course for the behavioral measurements of rotation in the 6-OHDA lesioned animals correlated with the phMRI. These experiments demonstrate that phMRI will become a valuable, noninvasive tool for investigation of neurotransmitter activity in vivo.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9339439&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1002/mrm.1910380306
dc.subjectAdrenergic Agents; Animals; Behavior, Animal; Brain; Cocaine; Dextroamphetamine; Dopamine; Dopamine Uptake Inhibitors; Magnetic Resonance Imaging; Microdialysis; Oxidopamine; Rats; Rats, Sprague-Dawley; Receptors, Dopamine; Sensitivity and Specificity; Tomography, Emission-Computed
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleDetection of dopaminergic neurotransmitter activity using pharmacologic MRI: correlation with PET, microdialysis, and behavioral data
dc.typeJournal Article
dc.source.journaltitleMagnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester)
dc.source.volume38
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/203
dc.identifier.contextkey586721
html.description.abstract<p>The metabolic activation resulting from direct dopaminergic stimulation can be detected using auto-radiography, positron emission tomography (PET) or, potentially, fMRI techniques. To establish the validity of the latter possibility, we have performed a number of experiments. We measured the regional selectivity of two different dopaminergic ligands: the dopamine release compound D-amphetamine and the dopamine transporter antagonist 2 beta-carbomethoxy-3 beta-(4-fluoropheny) tropane (CFT). Both compounds led to increased signal intensity in gradient echo images in regions of the brain with high dopamine receptor density (frontal cortex, striatum, cingulate cortex > > parietal cortex). Lesioning the animals with unilaterally administered 6-hydroxydopamine (6-OHDA) led to ablation of the phMRI response on the ipsilateral side; control measurements of rCBV and rCBF using bolus injections of Gd-DTPA showed that the baseline rCBV and rCBF values were intact on the lesioned side. The time course of the BOLD signal changes paralleled the changes observed by microdialysis measurements of dopamine release in the striatum for both amphetamine and CFT; peaking at 20-40 min after injection and returning to baseline at about 70-90 min. Signal changes were not correlated with either heart rate, blood pressure or pCO2. Measurement of PET binding in the same animals showed an excellent correlation with the phMRI data when compared by either measurements of the number of pixels activated or percent signal change in a given region. The time course for the behavioral measurements of rotation in the 6-OHDA lesioned animals correlated with the phMRI. These experiments demonstrate that phMRI will become a valuable, noninvasive tool for investigation of neurotransmitter activity in vivo.</p>
dc.identifier.submissionpathgsbs_sp/203
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages389-98


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