Methods to analyze novel liaisons between endosomes and centrosomes
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Student Authors
Hui-Fang HungUMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2015-09-12Keywords
AppendagesCenexin
Centriolin
Centrosome
Centrosome isolation
Evi5
Fip3
Mitosis
Mother centriole
Rab11
Rab8
Recycling endosome
Spindle pole
Cell Biology
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Show full item recordAbstract
For some time, it has been known that recycling endosomes (REs) are organized in a nebulous "pericentrosomal" region in interphase cells. However, the collective use of previously developed methods, including centrosome isolation, live cell imaging, and electron microscopy, suggested that there is much more going on between the centrosome and the RE than previously imagined. By exploiting these approaches, we uncovered novel roles of the centrosome in RE function and, conversely, novel roles for REs in centrosome function. We first found that REs dynamically localized to the centrosome throughout the cell cycle. More specifically, we found that REs interacted with appendages of the older centriole in interphase cells to control endosome recycling, and this interaction was governed by RE-machinery including the small GTPase Rab11. We next determined that REs carry centrosome proteins to spindle poles as part of the "centrosome maturation" process. Here we discuss the methods used and materials needed to complete these types of studies.Source
Methods Cell Biol. 2015;130:47-58. doi: 10.1016/bs.mcb.2015.03.024. Epub 2015 Jun 11. Link to article on publisher's site
DOI
10.1016/bs.mcb.2015.03.024Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33512PubMed ID
26360027Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/bs.mcb.2015.03.024