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dc.contributor.authorRanade, Koustubh
dc.contributor.authorPoteete, Anthony R.
dc.date2022-08-11T08:08:56.000
dc.date.accessioned2022-08-23T16:13:10Z
dc.date.available2022-08-23T16:13:10Z
dc.date.issued1993-08-01
dc.date.submitted2008-05-12
dc.identifier.citation<p>J Bacteriol. 1993 Aug;175(15):4712-8.</p>
dc.identifier.issn0021-9193 (Print)
dc.identifier.doi10.1128/jb.175.15.4712-4718.1993
dc.identifier.pmid8335629
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33558
dc.description.abstractThe superinfection exclusion gene (sieB) of Salmonella phage P22 was mapped with phage deletion mutants. The DNA sequence in the region was reexamined in order to find an open reading frame consistent with the deletion mapping. Several discrepancies with the previously published sequence were discovered. The revised sequence revealed a single open reading frame of 242 codons with six likely translation initiation codons. On the basis of deletion and amber mutant phenotypes, the second of these six sites was inferred to be the translation initiation site of the sieB gene. The sieB gene encodes a polypeptide with 192 amino acid residues with a calculated molecular weight of 22,442, which is in reasonable agreement with that estimated from polyacrylamide gels. The transcription start site of sieB was identified by the use of an RNase protection assay. The sieB promoter thus identified was inactivated by a 2-base substitution in its -10 hexamer. The sieB gene of coliphage lambda was also identified. The promoter for lambda sieB was identified by homology to that of P22 sieB.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8335629&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC204922/
dc.subjectAmino Acid Sequence; Bacteriophage P22; Bacteriophage lambda; Base Sequence; Gene Deletion; Genes, Viral; Molecular Sequence Data; Open Reading Frames; Promoter Regions (Genetics)
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleSuperinfection exclusion (sieB) genes of bacteriophages P22 and lambda
dc.typeJournal Article
dc.source.journaltitleJournal of bacteriology
dc.source.volume175
dc.source.issue15
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/23
dc.identifier.contextkey507279
html.description.abstract<p>The superinfection exclusion gene (sieB) of Salmonella phage P22 was mapped with phage deletion mutants. The DNA sequence in the region was reexamined in order to find an open reading frame consistent with the deletion mapping. Several discrepancies with the previously published sequence were discovered. The revised sequence revealed a single open reading frame of 242 codons with six likely translation initiation codons. On the basis of deletion and amber mutant phenotypes, the second of these six sites was inferred to be the translation initiation site of the sieB gene. The sieB gene encodes a polypeptide with 192 amino acid residues with a calculated molecular weight of 22,442, which is in reasonable agreement with that estimated from polyacrylamide gels. The transcription start site of sieB was identified by the use of an RNase protection assay. The sieB promoter thus identified was inactivated by a 2-base substitution in its -10 hexamer. The sieB gene of coliphage lambda was also identified. The promoter for lambda sieB was identified by homology to that of P22 sieB.</p>
dc.identifier.submissionpathgsbs_sp/23
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.source.pages4712-8


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