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dc.contributor.authorClemson, Christine Moulton
dc.contributor.authorMcNeil, John A.
dc.contributor.authorWillard, Huntington F.
dc.contributor.authorLawrence, Jeanne B.
dc.date2022-08-11T08:08:56.000
dc.date.accessioned2022-08-23T16:13:12Z
dc.date.available2022-08-23T16:13:12Z
dc.date.issued1996-02-01
dc.date.submitted2008-08-27
dc.identifier.citationJ Cell Biol. 1996 Feb;132(3):259-75. <a href="http://dx.doi.org/10.1083/jcb.132.3.259 ">Link to article on publisher's website</a>
dc.identifier.issn0021-9525 (Print)
dc.identifier.doi10.1083/jcb.132.3.259
dc.identifier.pmid8636206
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33568
dc.description.abstractThe XIST gene is implicated in X chromosome inactivation, yet the RNA contains no apparent open reading frame. An accumulation of XIST RNA is observed near its site of transcription, the inactive X chromosome (Xi). A series of molecular cytogenetic studies comparing properties of XIST RNA to other protein coding RNAs, support a critical distinction for XIST RNA; XIST does not concentrate at Xi simply because it is transcribed and processed there. Most notably, morphometric and 3-D analysis reveals that XIST RNA and Xi are coincident in 2- and 3-D space; hence, the XIST RNA essentially paints Xi. Several results indicate that the XIST RNA accumulation has two components, a minor one associated with transcription and processing, and a spliced major component, which stably associates with Xi. Upon transcriptional inhibition the major spliced component remains in the nucleus and often encircles the extra-prominent heterochromatic Barr body. The continually transcribed XIST gene and its polyadenylated RNA consistently localize to a nuclear region devoid of splicing factor/poly A RNA rich domains. XIST RNA remains with the nuclear matrix fraction after removal of chromosomal DNA. XIST RNA is released from its association with Xi during mitosis, but shows a unique highly particulate distribution. Collective results indicate that XIST RNA may be an architectural element of the interphase chromosome territory, possibly a component of nonchromatin nuclear structure that specifically associates with Xi. XIST RNA is a novel nuclear RNA which potentially provides a specific precedent for RNA involvement in nuclear structure and cis-limited gene regulation via higher-order chromatin packaging.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8636206&dopt=Abstract ">Link to article in PubMed</a>
dc.subject*Cell Cycle; Cell Line; Chromatin; Collagen; DNA Probes; Diploidy; Female; G1 Phase; Gene Library; Humans; In Situ Hybridization, Fluorescence; Male; Mitosis; Models, Structural; Nuclear Matrix; RNA; RNA Splicing; *RNA, Untranslated; Transcription Factors; Transcription, Genetic; *X Chromosome
dc.subjectCell Biology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleXIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure
dc.typeJournal Article
dc.source.journaltitleThe Journal of cell biology
dc.source.volume132
dc.source.issue3
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1238&amp;context=gsbs_sp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/239
dc.identifier.contextkey606037
refterms.dateFOA2022-08-23T16:13:13Z
html.description.abstract<p>The XIST gene is implicated in X chromosome inactivation, yet the RNA contains no apparent open reading frame. An accumulation of XIST RNA is observed near its site of transcription, the inactive X chromosome (Xi). A series of molecular cytogenetic studies comparing properties of XIST RNA to other protein coding RNAs, support a critical distinction for XIST RNA; XIST does not concentrate at Xi simply because it is transcribed and processed there. Most notably, morphometric and 3-D analysis reveals that XIST RNA and Xi are coincident in 2- and 3-D space; hence, the XIST RNA essentially paints Xi. Several results indicate that the XIST RNA accumulation has two components, a minor one associated with transcription and processing, and a spliced major component, which stably associates with Xi. Upon transcriptional inhibition the major spliced component remains in the nucleus and often encircles the extra-prominent heterochromatic Barr body. The continually transcribed XIST gene and its polyadenylated RNA consistently localize to a nuclear region devoid of splicing factor/poly A RNA rich domains. XIST RNA remains with the nuclear matrix fraction after removal of chromosomal DNA. XIST RNA is released from its association with Xi during mitosis, but shows a unique highly particulate distribution. Collective results indicate that XIST RNA may be an architectural element of the interphase chromosome territory, possibly a component of nonchromatin nuclear structure that specifically associates with Xi. XIST RNA is a novel nuclear RNA which potentially provides a specific precedent for RNA involvement in nuclear structure and cis-limited gene regulation via higher-order chromatin packaging.</p>
dc.identifier.submissionpathgsbs_sp/239
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages259-75
dc.contributor.studentChristine Clemson


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