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dc.contributor.authorFleckner, Jan
dc.contributor.authorZhang, Meng
dc.contributor.authorValcarcel, Juan
dc.contributor.authorGreen, Michael R.
dc.date2022-08-11T08:08:57.000
dc.date.accessioned2022-08-23T16:13:27Z
dc.date.available2022-08-23T16:13:27Z
dc.date.issued1997-07-15
dc.date.submitted2008-09-03
dc.identifier.citation<p>Genes Dev. 1997 Jul 15;11(14):1864-72.</p>
dc.identifier.issn0890-9369 (Print)
dc.identifier.doi10.1101/gad.11.14.1864
dc.identifier.pmid9242493
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33625
dc.description.abstractSplicing of mRNA precursors (pre-mRNAs) comprises a series of ATP-dependent steps, the first of which is the stable binding of U2 snRNP at the pre-mRNA branchpoint. The basis of ATP use for the interaction between U2 snRNP and the branchpoint is unclear, and, in particular, none of the known mammalian factors required for this step have the sequence characteristics of proteins that hydrolyze ATP. Entry of U2 snRNP into the spliceosome is initiated by interaction of the essential splicing factor U2AF65 with the pre-mRNA polypyrimidine tract. In this report we identify a new region of U2AF65 required for function, and use this information to clone a human 56-kD U2AF65 associated protein (UAP56). We show that UAP56 is an essential splicing factor, which is recruited to the pre-mRNA dependent on U2AF65, and is required for the U2 snRNP-branchpoint interaction. The sequence of UAP56 indicates it is a member of the DEAD box family of RNA-dependent ATPases, which mediate ATP hydrolysis during several steps of yeast pre-mRNA splicing. Our results reveal a new function of U2AF65: to position a DEAD box protein required for U2 snRNP binding at the pre-mRNA branchpoint region.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9242493&dopt=Abstract ">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1101/gad.11.14.1864
dc.subjectAdenosine Triphosphatases; Amino Acid Sequence; Hela Cells; Humans; Molecular Sequence Data; *Nuclear Proteins; Protein Binding; *RNA Splicing; RNA, Messenger; Ribonucleoprotein, U2 Small Nuclear; Ribonucleoproteins; Sequence Homology, Amino Acid; Spliceosomes
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleU2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction
dc.typeArticle
dc.source.journaltitleGenes and development
dc.source.volume11
dc.source.issue14
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/295
dc.identifier.contextkey616271
html.description.abstract<p>Splicing of mRNA precursors (pre-mRNAs) comprises a series of ATP-dependent steps, the first of which is the stable binding of U2 snRNP at the pre-mRNA branchpoint. The basis of ATP use for the interaction between U2 snRNP and the branchpoint is unclear, and, in particular, none of the known mammalian factors required for this step have the sequence characteristics of proteins that hydrolyze ATP. Entry of U2 snRNP into the spliceosome is initiated by interaction of the essential splicing factor U2AF65 with the pre-mRNA polypyrimidine tract. In this report we identify a new region of U2AF65 required for function, and use this information to clone a human 56-kD U2AF65 associated protein (UAP56). We show that UAP56 is an essential splicing factor, which is recruited to the pre-mRNA dependent on U2AF65, and is required for the U2 snRNP-branchpoint interaction. The sequence of UAP56 indicates it is a member of the DEAD box family of RNA-dependent ATPases, which mediate ATP hydrolysis during several steps of yeast pre-mRNA splicing. Our results reveal a new function of U2AF65: to position a DEAD box protein required for U2 snRNP binding at the pre-mRNA branchpoint region.</p>
dc.identifier.submissionpathgsbs_sp/295
dc.contributor.departmentProgram in Gene Function and Expression
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages1864-72


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