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    Kinetics of hairpin ribozyme cleavage in yeast

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    Authors
    Donahue, Christine Patricia
    Fedor, Martha J.
    UMass Chan Affiliations
    Department of Biochemistry and Molecular Biology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    1997-09-18
    Keywords
    Antisense Elements (Genetics); Base Sequence; Kinetics; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Phosphoglycerate Kinase; Poly A; RNA Caps; RNA, Catalytic; RNA, Messenger; Yeasts
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369543/
    Abstract
    Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast. Intracellular ribozyme-mediated cleavage occurs at similar rates and shows similar inhibition by ribozyme mutations as ribozyme-mediated reactions in vitro, but only when ribozymes are located in a favorable mRNA sequence context. The impact of cleavage on mRNA abundance is shown to depend directly on intrinsic mRNA stability. Surprisingly, cleavage products are no more labile than uncleaved mRNAs despite the loss of terminal cap structures or poly (A).
    Source

    RNA. 1997 Sep;3(9):961-73.

    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/33652
    PubMed ID
    9292496
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