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dc.contributor.authorDonahue, Christine Patricia
dc.contributor.authorFedor, Martha J.
dc.date2022-08-11T08:08:57.000
dc.date.accessioned2022-08-23T16:13:34Z
dc.date.available2022-08-23T16:13:34Z
dc.date.issued1997-09-18
dc.date.submitted2008-09-04
dc.identifier.citation<p>RNA. 1997 Sep;3(9):961-73.</p>
dc.identifier.issn1355-8382 (Print)
dc.identifier.pmid9292496
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33652
dc.description.abstractHairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast. Intracellular ribozyme-mediated cleavage occurs at similar rates and shows similar inhibition by ribozyme mutations as ribozyme-mediated reactions in vitro, but only when ribozymes are located in a favorable mRNA sequence context. The impact of cleavage on mRNA abundance is shown to depend directly on intrinsic mRNA stability. Surprisingly, cleavage products are no more labile than uncleaved mRNAs despite the loss of terminal cap structures or poly (A).
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9292496&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369543/
dc.subjectAntisense Elements (Genetics); Base Sequence; Kinetics; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Phosphoglycerate Kinase; Poly A; RNA Caps; RNA, Catalytic; RNA, Messenger; Yeasts
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleKinetics of hairpin ribozyme cleavage in yeast
dc.typeJournal Article
dc.source.journaltitleRNA (New York, N.Y.)
dc.source.volume3
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/319
dc.identifier.contextkey619031
html.description.abstract<p>Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast. Intracellular ribozyme-mediated cleavage occurs at similar rates and shows similar inhibition by ribozyme mutations as ribozyme-mediated reactions in vitro, but only when ribozymes are located in a favorable mRNA sequence context. The impact of cleavage on mRNA abundance is shown to depend directly on intrinsic mRNA stability. Surprisingly, cleavage products are no more labile than uncleaved mRNAs despite the loss of terminal cap structures or poly (A).</p>
dc.identifier.submissionpathgsbs_sp/319
dc.contributor.departmentDepartment of Biochemistry and Molecular Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages961-73


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