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dc.contributor.authorPuri, Vishwajeet
dc.contributor.authorKonda, Silvana
dc.contributor.authorRanjit, Srijana
dc.contributor.authorAouadi, Myriam
dc.contributor.authorChawla, Anil
dc.contributor.authorChouinard, My T.
dc.contributor.authorChakladar, Abhijit
dc.contributor.authorCzech, Michael P.
dc.date2022-08-11T08:08:57.000
dc.date.accessioned2022-08-23T16:13:48Z
dc.date.available2022-08-23T16:13:48Z
dc.date.issued2007-09-22
dc.date.submitted2008-09-11
dc.identifier.citationJ Biol Chem. 2007 Nov 23;282(47):34213-8. Epub 2007 Sep 19. <a href="http://dx.doi.org/10.1074/jbc.M707404200">Link to article on publisher's site</a>
dc.identifier.issn0021-9258 (Print)
dc.identifier.doi10.1074/jbc.M707404200
dc.identifier.pmid17884815
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33712
dc.description.abstractFat-specific protein (FSP)27/Cidec is most highly expressed in white and brown adipose tissues and increases in abundance by over 50-fold during adipogenesis. However, its function in adipocytes has remained elusive since its discovery over 15 years ago. Here we demonstrate that FSP27/Cidec localizes to lipid droplets in cultured adipocytes and functions to promote lipid accumulation. Ectopically expressed FSP27-GFP surrounds lipid droplets in 3T3-L1 adipocytes and colocalizes with the known lipid droplet protein perilipin. Immunostaining of endogenous FSP27 in 3T3-L1 adipocytes also confirmed its presence on lipid droplets. FSP27-GFP expression also markedly increases lipid droplet size and enhances accumulation of total neutral lipids in 3T3-L1 preadipocytes as well as other cell types such as COS cells. Conversely, RNA interference-based FSP27/Cidec depletion in mature adipocytes significantly stimulates lipolysis and reduces the size of lipid droplets. These data reveal FSP27/Cidec as a novel adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17884815&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1074/jbc.M707404200
dc.subject3T3 Cells; Adipocytes; Adipogenesis; Adipose Tissue, Brown; Adipose Tissue, White; Animals; COS Cells; Cercopithecus aethiops; Lipolysis; Male; Mice; Phosphoproteins; Proteins; RNA Interference; Triglycerides
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleFat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage
dc.typeJournal Article
dc.source.journaltitleThe Journal of biological chemistry
dc.source.volume282
dc.source.issue47
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/375
dc.identifier.contextkey626926
html.description.abstract<p>Fat-specific protein (FSP)27/Cidec is most highly expressed in white and brown adipose tissues and increases in abundance by over 50-fold during adipogenesis. However, its function in adipocytes has remained elusive since its discovery over 15 years ago. Here we demonstrate that FSP27/Cidec localizes to lipid droplets in cultured adipocytes and functions to promote lipid accumulation. Ectopically expressed FSP27-GFP surrounds lipid droplets in 3T3-L1 adipocytes and colocalizes with the known lipid droplet protein perilipin. Immunostaining of endogenous FSP27 in 3T3-L1 adipocytes also confirmed its presence on lipid droplets. FSP27-GFP expression also markedly increases lipid droplet size and enhances accumulation of total neutral lipids in 3T3-L1 preadipocytes as well as other cell types such as COS cells. Conversely, RNA interference-based FSP27/Cidec depletion in mature adipocytes significantly stimulates lipolysis and reduces the size of lipid droplets. These data reveal FSP27/Cidec as a novel adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation.</p>
dc.identifier.submissionpathgsbs_sp/375
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages34213-8


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